Methods of screening for compounds which inhibit the direct bind

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using a micro-organism to make a protein or polypeptide

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435 723, 435 691, 435 698, 435 699, 436501, 436 64, G01N 3353, G01N 33574

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active

055829953

ABSTRACT:
Disclosed is a method of screening for compounds which inhibit the direct binding of Ras or Raf-binding fragments thereof to Raf or Ras binding fragments thereof.

REFERENCES:
Fields et al. Nature 340 pp. 245-246 (1989) "A Novel Genetic System to Detect Protein--Protein Interactions".
Luban et al. J. Virol. 66 #8 pp. 5157-5160 (Aug. 1992) "Genetic Assay for Multimerization of Retroviral Gag Polyproteins".
Berg J. B. C. 265 #12 pp. 6513-6516 (1990) "Zinc Fingers and Other Metal-binding Domains".
Mulcahy et al. Nature 313 pp. 241-243 (1985) "Requirement for Gas Proto-Oncogene Function During Serum-Stimulated Growth of NIH NT3 Cells".
Rocquigny et al. Biochem. Biocphys. Res. Comm. 180 #2 pp. 1010-1016 (1991) "First Large Scale Chemical Synthesis of the 72 Amino Acid HIV-1 Nucleocapsid Protein NC p. 7 in an Active Form".
Zhang et al. Nature 364 pp. 308-313 (Jul. 1993) "Normal and Oncogenic p21.sup.(a) Proteins Bind to the Amino Terminal Regulatory Domain of c-Raf-1".
Dermer, Bio/Technology, vol. 12 Mar. 1994, p. 320 "Another Anniversary for the War on Cancer".
Plattner et al., "Obstacles to drug development from peptide leads", from Drug Discovery Technologies, Ed. by Clark et al., Ellis Harwodd Ltd. (1990), pp. 92-126.
TIG, vol. 10, #2, pp. 45-48 (1994) "The 3Rs of life: Ras, Raf and growth regulation".
Bonner et al.; The complete coding sequence of the human raf oncogene and the corresponding structure of the c-raf-1 gene; Nucleic Acids Research; 14: 1009-1015 (1986).
Bruder et al.; Serum-, TPA-, and Ras-induced expression from Ap-1/Ets-driven promoters requires Raf-1 kinase; Genes & Development; 6:545-556 (1992).
Chien et al.; The two-hybrid system: A method to identify and clone genes for proteins that interact with a protein of interest; Proc. Natl. Acad. Sci. USA; 88:9578-9582 (1991).
Durfee et al.; The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit; Genes & Development; 7:555-569 (1993).
Guan et al.; Eukaryotic Proteins Expressed in Escherichia coli: An Improved Thrombin Cleavage and Purification Procedure of Fusion Proteins with Glutathione S-Transferase; Analytical Biochemistry; 192:262-267.
Heidecker et al.; The Role of Raf-1 Phosphorylation in Signal Transduction; Viral Pathology Section; Laboratory of Viral Carcinogenesis; NCI-Frederick Cancer Research and Development Center, Frederick, Maryland 21702.
Howe et al.; Activation of the MAP Kinase Pathway by the Protein Kinase raf; Cell; 71:335-342 (1992).
Huleihel et al.; Characterization of Murine A-raf, a New Oncogene Related to the v-raf Oncogene; Molecular and Cellular Biology; 6:2655-2662 (1986).
Itoh et al.; The Post-translational Processing of rasp21 Is Critical for Its Stimulation of Mitogen-activated Protein Kinase; The Journal of Biological Chemistry; 268:3025-3028 (1993).
Kitayama et al.; Genetic analysis of the Kirsten-ras-revertant 1 gene: Potentiation of its tumor suppressor activity by specific point mutations; Proc. Natl. Acad. Sci. USA; 87:4284-4288 (1990).
Kolch et al.; Raf-1 protein kinase is required for growth of induced NIH/3T3 cells; Nature; 349:426-428 (1991).
Kovacina et al.; Insulin Activates teh Kinase Activity of the Raf-1 Proto-oncogene by Increasing Its Serine Phosphorylation; The Journal of Biological Chemistry; 265:12115-12118 (1990).
Kyriakis et al.; Raf-1 activates MAP kinase--kinase; Nature; 358:417-421 (1992).
Marshall et al.; Characterization of Ras effector mutant interactions with the NF1-GAP related domain; Oncogene; 8:425-431 (1993).
Marshall et al.; Identification of Amino Acid Residues Required for Ras p21 Target Activation; Molecular and Cellular Biology; 11:3997-4004 (1991).
Mizuno et al.; A stimulatory GDP/GTP exchange protein for smg p21 is active on the post-translationally processed form of c-Ki-ras p21 and rhoA p21; Proc. Nat. Acad. Sci. USA; 88:6442-6446 (1991).
Quilliam et al.; Biochemical Characterization of Baculovirus-Expressed rap1A/Krev-1 and Its Regulation by GTPase-Activating Proteins; Molecular and Cellular Biology; 10:2901-2908 (1990).
Satoh et al.; Function of Ras as a Molecular Switch in Signal Transduction; The Journal of Biological Chemistry; 267:24149-24152 (1992).
Stanton et al.; Definition of the Human raf Amino-Terminal Regulatory Region by Deletion Mutagenesis; Molecular and Cellular Biology; 9:639-647 (1989).
Tsuda et al.; A Protein Kinase Similar to MAP Kinase Activator Acts Downstream of the Raf Kinase in Drosophila; Cell; 72:407-414 (1993).
Valencia et al.; The ras Protein Family: Evolutionary Tree and Role of Conserved Amino Acids; Biochemistry; 30:4637-4647 (1991).

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