Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1997-09-15
2000-02-01
Gitomer, Ralph
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 21, C12Q 118
Patent
active
060201495
ABSTRACT:
A method for determining the anti-microbial activity of a putative anti-microbial agent includes combining a microbially required nucleotide phosphatase, a nucleoside phosphate and the substance to be tested, and assessing the extent of degradation of the nucleoside phosphate in the presence and absence of the substance. The method thus allows the determination of the extent of inhibition of the nucleotide phosphatase by the substance. Preferably the method determines the degree of inhibition of an RNA helicase such as DbpA, which acts selectively on prokaryotic ribosomal RNA. Suitable DbpA inhibitors as well as genetic material encoding for an active form of DbpA are used.
REFERENCES:
patent: 4416995 (1983-11-01), Amaral
patent: 5466576 (1995-11-01), Schulz et al.
Nature, vol. 337, No. 6203, Jan. 12, 1989, pp. 121-122, P. Linder et al.
Nucleic Acids Research, vol. 18, No. 18, Sep. 25, 1990, pp. 5413-5417, R. Iggo et al, "Identification of a putative RNA helicase in E. coli".
Biological Abstracts, vol. 77, No. 9, 1984, Abstract No. 72366, S. W. Matson et al., DNA-dependent nucleoside 5-triphosphatase activity of gene 4 protein . . .
Chemical Abstracts, vol. 117, No. 19, Nov. 9, 1992, abstract No. 184427, N. R. Bachur et al. "Helicase inhibition by anthracycline anticancer agents".
Biochemistry, vol. 31, No. 10, 1992, pp. 2822-2829, D. Sun et al., "Effect of the (+)-CC-1065-(N3-adenine)DNA adduct on in vitro DNA synthesis mediated by . . . ".
Journal of Biological Chemistry, vol. 258, No. 22, Nov. 25, 1983, pp. 14009-14016, Matson et al, "DNA-dependent Nucleoside 5'-Triphosphatase Activity . . . ".
Molecular Pharmacology, 41:993-998, Dec, 31, 1991, Bachur et al, "Helicase Inhibition by Anthracycline Anticancer Agents".
Journal of Biological Chemistry, vol. 258, No. 22, Nov. 25, 1983, pp. 14017-14024, Matson et al, "The Gene 4 Protein of Bacteriophage T7".
The New Biologist, vol. 3, No. 9, Sep. 1991, pp. 886-895, Kalman et al, "rhlB, A New Escherichia coli K-12 Gene with an RNA Helicase-Like Protein Sequence Motif . . . ".
Kalman M., rhlB, A New E. coli K-12 Gene With an RNA Helicase Like Protein Sequence Motif, One of at Least Five Such Possible Genes in a Prokaryote, The New Biologist 3(9) 886-895, Sep. 1991.
Fuller-Pace F., RNA Helicases, Nucleic Acids and Molecular Biology vol. 6 159-173, 1992.
Bachur N., Helicase Inhibition by Anthracycline Anticancer Agents, American Soc for Pharm and Exp Ther vol. 41 993-998, 1992.
Fuller-Pace Frances Victoria
Lane David Philip
Gitomer Ralph
The University Court of The University of Dundee
LandOfFree
Methods of screening for anti-microbial agents and for inhibitin does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods of screening for anti-microbial agents and for inhibitin, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods of screening for anti-microbial agents and for inhibitin will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-936378