Methods of screening apoptosis modulating compounds,...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007240, C435S007400, C435S377000

Reexamination Certificate

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11140275

ABSTRACT:
A method of screening cellular polypeptides for pro-apoptotic or anti-apoptotic activity in a cell of a particular cell-type by: (a) culturing cells of the particular cell-type under non apoptotic conditions and culturing cells of the particular cell-type under apoptotic conditions, and (b) determining subcellular localization of the cellular polypeptides in the cultured cells, wherein a localization of a cellular polypeptide in lipid rafts in cultured cells under non apoptotic conditions and a segregation of the cellular polypeptide from lipid rafts in cultured cells under apoptotic conditions is indicative that the cellular polypeptide has a pro-apoptotic or an anti-apoptotic activity in the particular cell-type.

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patent: WO 00/37088 (2000-06-01), None
patent: WO 01/10888 (2001-02-01), None
patent: WO 01/48236 (2001-07-01), None
Urbano A, Gorgun G, Foss F. Mechanisms of apoptosis by the tyrphostin AG957 in hematopoietic cells. Biochem Pharmacol. Feb. 15, 2002;63(4):689-92.
Gajate Consuelo et al: “The antitumor ether lipid ET-18-0CH3 induces apoptosis through translocation and capping of Fas/CD95 into membrane rafts in human leukemic cells.” BLOOD, vol. 98, No. 13. Dec. 15, 2001, pp. 3860-3863, XP002233039 Dec. 25, 2001, ISSN: 006-4971.
Kabouridis Panagiotis S et al: “Cholesterol depletion disrupts lipid rafts and modulates the activity of mulitple singaling pathways in T lymphocytes.” European Journal of Immunology., vol. 30, No. 3, Mar. 2000, pp. 954-963, XP002233040, issn:0014-2980.
Ayllon Veronica et al: “Protein phosphatase Ialpha is a Ras-activated Bad phosphatase that regulates interleukin-2 deprivation-induced apoptosis,” EMBO (European Molecular Biology Organization) Jountal, vol. 19, n0. 10, May 15, 2000, pp. 2237-2246, XP002233041, ISSN: 0261-4189.
Robollo A et al: “The association of Aiolos transcription factor and Bc1-xL is involved in the control of apoptosis.” Journal of Immunology (Baltimore, MD.: 1950) Unites States Dec. 1, 2001, vol. 167, No. 11, Dec. 1, 2001, pp. 6366-6373, XP002233042, ISSN: 0022-1767.
Simons K et al: “Lipid rafts and signal transduction” Nature reviews molecular cellbiology, Macmillan magazines, London, GB, vol. 1, No. 1, Oct. 2000, pp. 31-39, XP001006200.

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