Methods of screening an agent for an activity in an isolated...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

Reexamination Certificate

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C800S020000, C424S009200

Reexamination Certificate

active

07897363

ABSTRACT:
The present invention provides methods of screening an agent for an activity in an isolated organ, e.g., eye, from a teleost, e.g., zebrafish. Methods of isolating eyes from zebrafish are provided. Methods of screening an agent for an ocular activity in the isolated eye are provided. Methods of screening an agent for an ocular activity in a model of ocular disease or disorder are provided. Methods of screening an agent for an ocular activity in the isolated eye and for screening the agent for cell death and/or toxic activity in the eye or other organ or tissue are provided. The invention further provides high throughput methods of screening agents for an activity in isolated eyes of zebrafish in multi-well plates.

REFERENCES:
patent: 6656449 (2003-12-01), Serbedzija et al.
patent: 7176189 (2007-02-01), Baranowitz
patent: 2002/0187921 (2002-12-01), Rubeinstein
patent: 2005/0079577 (2005-04-01), Ashkenazi et al.
patent: 2006/0123487 (2006-06-01), Serbedzija et al.
PCT International Search Report and Written Opinion of Aug. 20, 2008 for Application No. PCT/US2008/066760.

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