Methods of sandwich hybridization for the quantitative analysis

Details Methods of sandwich hybridization for the quantitative analysis Methods of sandwich hybridization for the quantitative analysis

1991-07-30

1996-09-17

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 75, 435 25, 435 27, 435 28, 435 29, 435 34, 435 35, 536 221, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 935 77, 935 78, C12Q 168

Patent

active

055567487

ABSTRACT:
The invention is directed to a sandwich hybridization assay wherein a nucleic acid capture probe is firstly immobilized on an assay plate via masked receptors on the plate. The capture probe is immobilized by the binding of receptor ligands on the capture probe during this first step. Subsequently, target nucleic acid is hybridized to the immobilized capture probe either before or after the hybridization of an indicator nucleic acid probe onto the target. The target is quantified via detection of the immobilized indicator signal.

REFERENCES:
patent: 4486539 (1984-12-01), Ranki et al.
patent: 4563419 (1986-01-01), Ranki et al.
patent: 4582810 (1986-04-01), Rosenstein
patent: 5126241 (1992-06-01), Schenk
patent: 5374524 (1994-12-01), Milles
Syvanen Medical Biol. 64:313 (1986).
Ranki et al. Gene 21:77 (1983).
Syvanen et al. Nucleic Acids Res. 14(12):5037 (1986).
Urdea et al. Gene 61:253 (1987).

Affiliated with

Also associated with

Rating

Methods of sandwich hybridization for the quantitative analysis does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods of sandwich hybridization for the quantitative analysis , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods of sandwich hybridization for the quantitative analysis will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-412423