Methods of regulating protein glycosylation

Details Methods of regulating protein glycosylation Methods of regulating protein glycosylation

1988-05-03

1992-08-04

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 692, 435 696, 435255, 435256, 935 50, 935 69, C12N 1500, C12N 1581, C12N 119, C12P 2102

Patent

active

051358549

ABSTRACT:
Methods for producing a heterologous protein or polypeptide are disclosed. A preferred method utilizes a fungal cell carrying a defect in a gene whose product is required for the addition of outer chain oligosaccharide moieties to glycoproteins, the cell transformed with a first DNA construct comprising a regulated promoter followed downstream by a DNA sequence which complements the defect, and a second DNA construct comprising a second promoter followed downstream by a DNA sequence encoding a secretion signal and a DNA sequence encoding a heterologous protein or polypeptide. A yeast cell having a Mnn9.sup.- phenotype and capable of producing colonies of normal morphologies in the absence of osmotic stabilization is also disclosed.

REFERENCES:
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