Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Culture – maintenance – or preservation techniques – per se
Patent
1995-06-30
1998-03-24
Fox, David T.
Chemistry: molecular biology and microbiology
Plant cell or cell line, per se ; composition thereof;...
Culture, maintenance, or preservation techniques, per se
4354301, 800DIG24, A01H 400
Patent
active
057312028
ABSTRACT:
The invention relates to improved transformation and regeneration of alfalfa, Medicago sativa.
A method is used to transform alfalfa by using particle acceleration. Optimum results with mature cotyledons occurs when bombarding after 24 to 120 hours of imbibing water. Regeneration and transformation of alfalfa is greatly improved by using immature cotyledons or embryos of immature cotyledons for transformation and regeneration. Immature cotyledons include those up to about 25 days past pollination, and preferably include cotyledons excised at 10-15 days past pollination, most preferably including those excised at about 10 days past pollination. These cotyledons have a light green to translucent appearance. Plants resulting from bombardment of somatic embryos of immature cotyledons retain regeneration ability.
REFERENCES:
Brown, D., and A. Atanassov. Role of genetic background in somatic embryogenesis in Medicago. 4:111-122, 1985.
Hazra, S., S. Sathaye, and A. Mascarenhas. Direct somatic embryogenesis in peanut (Arachis hypogea). Biotechnology. 7:949-951, 1989.
Buising Charisse Marie
Tomes Dwight
Fox David T.
Kimball Melissa L.
Pioneer Hi-Bred International , Inc.
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