Methods of regeneration of medicago sativa and expressing foreig

Details Methods of regeneration of medicago sativa and expressing foreig Methods of regeneration of medicago sativa and expressing foreig

1995-06-30

1999-11-30

Robinson, Douglas W.

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

Involving particle-mediated transfecion

800205, 800DIG24, 435470, A01H 104, C12N 1500

Patent

active

059946261

ABSTRACT:
The invention relates to improved transformation and regeneration of alfalfa, Medicago sativa.
A method is used to transform alfalfa by using particle acceleration. Optimum results with mature cotyledons occurs when bombarding after 24 to 120 hours of imbibing water. Regeneration and transformation of alfalfa is greatly improved by using immature cotyledons or embryos of immature cotyledons for transformation and regeneration. Immature cotyledons include those up to about 25 days past pollination, and preferably include cotyledons excised at 10-15 days past pollination, most preferably including those excised at about 10 days past pollination. These cotyledons have a light green to translucent appearance. Plants resulting from bombardment of somatic embryos of immature cotyledons retain regeneration ability.

REFERENCES:
patent: 5015580 (1991-05-01), Christou et al.
patent: 5324646 (1994-06-01), Buising et al.
Brown, D.C.W., and A. Atanassov. Role of genetic background in somatic embryogenesis in Medicago. Plant Cell Tissue Organ Culture. 4:111-122, 1985.
Deak, M., G.B. Kiss, C. Koncz, and D. Dudits. Transformation of Medicago by Agrobacterium mediated transfer. Plant Cell Reports. 5:97-100, 1986.
Hazra, S., S.S. Sathaye, and A.F. Mascarenhas. Direct somatic embryogenesis in peanut (Arachis hypogea). Biotechnology. 7:949-951, 1989.
Miki, B.L., et al., 1993 "Procedures for Introducing Foreign DNA into Plants", Methods in Plant Molecular Biology and Biotechnology 67-88.
Desagagnes, R., et al., 1995 "Genetic Transformation of Commercial Breeding Lines of Alfalfa (Medicago sativa)", Plant Cell Tiss. Org. Cult. 42: 129-140.
M. Chabaud et al. Plant Cell Reports, vol. 7 ('88) pp. 512-516.

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