Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Reexamination Certificate
2006-03-14
2006-03-14
Tate, Christopher R. (Department: 1654)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
C930S090000
Reexamination Certificate
active
07012130
ABSTRACT:
The present invention relates, in general, to a method of purifying recombinant human erythropoietin (EPO). The present invention also relates to a substantially pure EPO. The method comprises a differential precipitation, an hydrophobic interaction chromatography, various concentration and diafiltration steps, tandem anionic and cationic exchange chromatographies and molecular exclusion chromatography for the obtaining of pure EPO. The method does not comprise high performance liquid chromatography steps. The invention also comprises the EPO obtained according to the claimed procedure.
REFERENCES:
patent: 4667016 (1987-05-01), Lai et al.
patent: 4667195 (1987-05-01), Hewick et al.
patent: 4703008 (1987-10-01), Lin
patent: 4806524 (1989-02-01), Kawaguchi et al.
patent: 5010002 (1991-04-01), Levinson et al.
patent: 5547933 (1996-08-01), Lin
patent: 5618698 (1997-04-01), Lin
patent: 5688679 (1997-11-01), Powell
patent: 5756349 (1998-05-01), Lin
patent: 5783559 (1998-07-01), Florin-Robertsson et al.
patent: WO 86/03520 (1986-06-01), None
patent: WO 92/06116 (1992-04-01), None
patent: WO 98/00530 (1998-01-01), None
patent: WO 00/27419 (2000-05-01), None
patent: WO 00/27869 (2000-05-01), None
patent: WO 00/27997 (2000-05-01), None
patent: WO 00/28066 (2000-05-01), None
Miyake et al. Purification of Human Erythropoietin; Journal of Biological Chemistry, vol. 252, No. 15 (1977), pp. 5558-5564.
Wojchowksi et al. Site-Specific Antibodies to Human Erythropoietin: Immunoaffinity Purifaction of Urinary and Recombinant Hormone; Biochimica et Biophysica Act-Protein Structure and Molecular Enzymology (1987), 913/2 pp. 170-178.
Andersen, D.C. and Goochee, C.F., “The effect of cell-culture conditions on the oligosaccharide structures of secreted glycoprotein,”Curr. Op. Biotech. 5:546-549, Current Biology Ltd. (1994).
Bondurant, M.C. and Koury, M.J., “Anemia Induces Accumulation of Erythropoietin mRNA in the Kidney and Liver,”Mol. Cell. Biol. 6:2731-3, American Society for Microbiology (1986).
Borsook, H., et al., “Polycythemic Response in Normal Adult Rats to a Nonprotein Plasma Extract from Anemic Rabbits,”Blood 9:734-742, Grune and Stratton, Inc. (1954).
Bowie, J.U., et al., “Deciphering the Message in Protein Sequences: Tolerance to Amino Acid Substitutions,”Science 247:1306-1310, American Association for the Advancement of Science (1990).
Brown, P.R. and Krstulovic, A.M., “Practical Aspects of Reverse-Phase Liquid Chromatography Applied to Biochemical and Biomedical Research,”Anal. Biochem. 99:1-21, Academic Press, Inc. (1979).
Burg, J., et al., “Human erythropoietin recombinant production by fermentation and protein purification using a series of chromatographic steps,”Chem. Abstracts 126:315 Abstract No. 57095j, American Chemical Society (1997).
Busuttil, R.W., et al., “The Cytological Localization of Erythropietin in the Human Kidney Using the Fluorescent Antibody Technique,”Proc. Soc. Exp. Biol. Med. 137:327-330, Academic Press, Inc. (1971).
Busuttil, R.W., et al., “Localization of Erythropoietin in the Glomerulus of the Hypoxic Dog Kidney Using a Fluorescent Antibody Technique,”Acta Haemat. 47:238-242, S. Karger (1972).
Carnot, M.P. and Deflandre, C., “Sur l'activité hémop{overscore ( i)}étique 'du sérum au cours de la regénération du sang,”C.R. Acad. Sci. 143:384-386, (1906).
Carnot, M.P. and Deflandre, C., “Sur l'activité hémopīétique des différents organes au cours de la regénération du sang,”C.R. Acad. Sci. 143:432-435 (1906).
Carnot, M.P., “Sur le Mécanisme de L'hyperglobulie Provoquée par le Sérum D'animaux en Rénovation Sanguine,”C.R.H. Sean. Mem. Soc. Biol. 111:344-346, Libraires de L'Académie de Médecine (1906).
Carnot, M.P., “Sur L'activité Cytopoiétique du Sang et des Organes Régénérés au Cours des Régénérations Viscérales,”C.R.H. Sean. Mem. Soc. Biol. 111:463-465, Libraires de L'Académie de Médecine (1906).
Caro, J. and Erslev, A.J., “Biologic and immunologic erythropoietin in extracts from hypoxic whole rat kidneys and in their glomerular and tubular fractions,”J. Lab. Clin. Med 103:922-931, C.V. Mosby Company (1984).
Caro, J., et al., “Erythropoietin Production by an Established Kidney Proximal Tubule Cell Line (LLCPK1) ,”Exp. Hematol 12:357, Springer-Veriag (1984).
Caro, J., et al., “Erythropoietin in liver tissue extracts and in liver perfusates from hypoxic rats,”Am. J. Physiol. 244:E431-E434, American Physiological Society (1983).
Dinkelaar, R.B., et al., “Metabolic Studies on Erythropoietin (Ep): II. The Role of Liver and Kidney in the Metabolism of Ep,”Exp. Hematol. 9:796-803, Allen Press, Inc. (1981).
Dornfest, B.S., et al., “Hepatic production of erythropoietin in a phenylhydrazine-induced compensated hemolytic state in the rat,”J. Lab. Clin. Med. 102:274-285, C.V. Mosby Company (1983).
Dornfest, B.S., et al., “Recovery of an Erythropoietic Inducing Factor from the Regenerating Rat Liver,”Ann. Clin. Lab. Sci. 11:37-46, Institute for Clinical Science (1981).
Erslev, A., “Humoral Regulation of Red Cell Production,”Blood 8:349-357, Grune and Stratton, Inc. (1953).
Erslev, A.J., “In Vitro Production of Erythropoietin by Kidneys Perfused With a Serum-free Solution,”Blood 44:77-85, Grune and Stratton, Inc. (1974).
Erslev, A.J. and Caro, J., “Physiologic and Molecular Biology of Erythropoietin,”Med. Oncol. Tumor Pharmacother. 3:159-164, Pergamon Press (1986).
Eschbach, J.W., et al., “Correction of Anemia of End-Stage Renal Disease with Recombinant Human Erythropoietin,”New Eng. J. Med. 316:73-78, Massachusetts Medical Society (1987).
Fisher, J.W. and Birdwell, B.J., “The Production of an Erythropoietic Factor by the In Situ Perfused Kidney,”Acta. Haemat. 26:224-232, S. Karger (1961).
Fisher, J.W., et al., “Localization of Erythropoietin in Glomeruli of Sheep Kidney by Fluorescent Antibody Technique,”Nature 205:611-612, Macmillan Magazines Ltd. (1965).
Fisher, J.W., “Pharmacologic Modulation of Erythropoietin Production, ”Ann. Rev. Pharmacol. Toxicol. 28:101-122, Annual Reviews, Inc. (1988).
Frenkel, E.P., et al., “Some Observations on the Localization of Erythropoietin,”Ann. N.Y. Acad. Sci. 149:292-293, New York Academy of Sciences (1968).
Gordon, A.S., et al., “A Plasma Extract with Erythropoietic Activity,”Proc. Soc. Exp. Biol. Med. 86:255-258, Society for Experimental Biology and Medicine (1954).
Han, F., et al., “Cloning of a human erythropoietin cDNA and its expression in COS-7 cells,”Chem. AbstractsAbstract No. 511352 (1996).
Hodgson, G. and Tohá, J., “The Erythropoietic Effect of Urine and Plasma of Repeatedly Bled Rabbits,”Blood 9:299-309, Grune and Stratton, Inc. (1954).
Jacobs, K., et al., “Isolation and characterization of genomic and cDNA clones of human erythropoietin,”Nature 313:806-810, Macmillan Magazines Ltd. (1985).
Jacobson, L.O., et al., “Role of the Kidney in Erythropoiesis,”Nature 179:633-634, Macmillan Magazines Ltd. (1957).
Jelkmann, W., et al., “Extraction of Erythropoietin from Isolated Renal Glomeruli of Hypoxic Rats,”Exp. Hematol. 11:581-588, Allen Press, Inc. (1983).
Jixian, D., et al., “Study on a serum-free medium used for production of rHuEPO,”Bull. Acad. Mil. Med. Sci. 21:244-246, Academy of Military Medical Sciences (1997).
Kazal, L.A. and Erslev, A.J., “Erythropoietin Production in Renal Tumors,”Ann. Clin. Lab. Sci. 5:98-109, Institute for Clinical Science (1975).
Krane, N.K., “The Role of Erythropoietin in the Anemia of Chronic Renal Failure,”H. Ford Hosp. Med. J. 31:177-181, Henry Ford Hospital
Carcagno Carlos Miguel
Criscuolo Marcelo Eduardo
Melo Carlos Alberto
Vidal Juan A.
Sterne Kessler Goldstein & Fox P.L.L.C.
Sterrenbeld Biotechnologie North America, Inc.
Tate Christopher R.
LandOfFree
Methods of purifying recombinant human erythropoietin from... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods of purifying recombinant human erythropoietin from..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods of purifying recombinant human erythropoietin from... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3555134