Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1998-03-18
2000-12-12
Yucel, Remy
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, C12Q 168
Patent
active
061596884
ABSTRACT:
A method for the construction of a library of recombined polynucleotides from a number of different starting single or double stranded parental DNA templates is disclosed, wherein the starting single or double stranded parental DNA templates represent discrete points in a population of genes encoding evolutionary or synthetic homologues of a peptide having homologies ranging over a broad spectrum from less than 15% to more than 80%, said population exhibiting at least one identification sequence, and whereby said genes are subjected to a gene shuffling procedure to generate shuffled mutants of said population of genes representing additional discrete points between those of said starting templates.
REFERENCES:
patent: 4518584 (1985-05-01), Mark et al.
patent: 4894331 (1990-01-01), Ratzkin et al.
patent: 5093257 (1992-03-01), Gray
patent: 5965408 (1999-08-01), Short
Crameri et al., Nature, vol. 391, pp. 288-291 (Jan. 15, 1998).
Stemmer, Nature, vol. 370, No. 4, pp. 389-391 (Aug. 4, 1994).
Weber et al., Nucleic Acids Research, vol. 11, No. 16, pp. 5661-5669 (1983).
Pompon et al. Gene. vol. 83, pp. 15-25, 1989.
Stemmer, W.P.C. Proceedings of the National Acad. of Sciences, USA. vol. 91, pp. 10747-10751, Oct. 1994.
Borchert Torben Vedel
Cherry Joel R.
Kretzschmar Titus
Vind Jesper
Gregg Valete
Novo Nordisk A S
Novo Nordisk Biotech Inc.
Yucel Remy
Zelson Steve T.
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