Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Reexamination Certificate
2007-10-22
2010-10-05
Nashed, Nashaat T (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
C435S232000, C435S183000, C435S155000, C435S156000
Reexamination Certificate
active
07807424
ABSTRACT:
The present invention comprises crystalline polyketide synthases, isolated non-native polyketide synthases having the structural coordinates of said crystalline polyketide synthases, and nucleic acid encoding such non-native polyketide synthases. Also disclosed are methods of producing mutant polyketide synthases, and methods of altering the activity and/or substrate specificity of putative polyketide synthases.
REFERENCES:
patent: 6265633 (2001-07-01), Okada et al.
patent: 6390821 (2002-05-01), Shokat
patent: WO 01/07579 (2001-02-01), None
Alignment of Seq Id No. 1 (Qy) with Db Uniports, Accession No. 023882 (Jul. 1999).
Alignment of Seq Id No. 1 (Qy) with Db Uniports, Accession No. 023883 (Jul. 1999).
Suh et al., “Identification of amino acid residues important in the cyclization reactions of chalcone and stilbene synthases.” Biochem. J., 350: 229-235, 2000.
International Search Report for PCT Application No. PCT/US01/48523.
Eckermann et al., “New pathway to polyketides in plants.”Nature396:387-390, (1998).
Ferrer et al., “Chalcone synthase from alfalfa.” Database for ′Online!, Id 1BI5, Jun. 22, 1999.
Ferrar et al., “Structure of chalcone synthase and the molecular basis of plant polyketide biosynthesis.”Nature Structural Biology, 8: 775-784, 1999.
He et al., “Structural modeling and site-directed mutagenesis of the actinorhodin β-ketoacyl-acyl carrier protein synthase.”Journal of Bacteriology, 182: 2619-1623, 2000.
Helariutta et al., “Chalcone synthase-like genes active during corolla development are differently expressed and encode enzymes with different catalytic properties inGerbera hybrida(Asteraceae).”Plant Molecular Biology, 28: 47-60, 1995.
Huang et al., “Crystal structure of β-ketoacyl-acyl carrier protein synthase II fromE. colireveals the molecular architecture of condensing enzymes.”The Embo Journal, 17: 1183-1191, 1998.
Jez et al., “Dissection of malonyl-coenzyme A decarboxylation from polyketide formation in the reaction mechanism of a plant polyketide synthase.”Biochemistry, 39:890-902, 2000.
Jez et al., “Structure of chalcone synthase and the molacular basis of plant ppolyketide biosynthesis.” Meeting information: Annual Meeting of the American Societies for Experimental Biology on Biochemistry and Molecular Biology, San Francisco, CA., May 16-20, 1999.FASEB Journal: vol. 13, No. 7, p. A1392, XP002158823, Abstract 355.
Junghans et al., “Stress responses in alfalfa (Medicago sativaL.) 15. Characterization and expression patterns of members of a subset of the chalcone synthase multigene family.”Plant Mol. Biol., 22:239-253, (1993).
Kreuzaler and Hahlbrock, “Enzymic synthesis of an aromatic ring from acetate units: Partial purification and some properties of flavanone synthase from cell-suspension cultures ofpetroselinum hortense.” Eur. J. Biochem., 56:205-213, (1975).
Lanz et al., “The role of cysteines in polyketide synthases: Site-directed mutagenesis of resveratrol and chalcone synthases, two key enzymes in different plant-specific pathways.”J.Biol.Chem. 266:9971-9976, (1991).
Mathieu et al., “The 2.8 Å crystal structure of peroxisomal 3-ketoacyl-CoA thiolase ofSaccharomyces cerevisiae: a five-layered αβαβα structure constructed from two core domains of identical topology.”Structure, 2:797-808, (1994).
Otwinowski and Minor, “Processing of X-ray diffraction data collected in oscillation mode.”Methods Enzymol. 276:307-26, (1997).
Preisig-Mueller et al., “Plant polyketide synthases leading to stilbenoids have a domain catalyzing malonyl-CoA:CO2exchange, malonyl-CoA decarboxylation, and covalent enzyme modification and a site for chain lengthening.”Biochemistry36:8349-8358, (1997).
Raiber et al., “Molecular and enzymatic characterization of two stilbene synthases from eastern white pine (Pinus strobus): A single Arg/His difference determines the activity and the pH dependence of the enzymes.”FEBS Letters, 361: 299-302, 1995.
Schroder, “A family of plant-specific polyketide synthases: facts and predictions.”Trends in Plant Science, 2: 373-378, 1997.
Schroeder et al., “Plant polyketide synthases: a chalcone synthase-type enzyme which performs a condensation reaction with methylmalonyl-CoA in the biosynthesis of C-methylated chalcones.”Biochemistry, 37:8417-8425, (1998).
Schroder and Schroder, “Stilbene and Chalcone synthases: Related enzymes with key functions in plant-specific pathways.” Zeitschrift Fuer Naturforschung. Section C.Biosciences, 45c: 1-8, 1990.
Tropf et al., “Reaction mechanisms of homodimeric plant polyketide synthases (stilbene and chalcone sysnthase).”J.Biol.Chem., 270:7922-7928, (1995).
Welle & Grisebach, “Isolation of a novel NADPH-dependent reductase which coacts with chalcone synthase in the biosynthesis of 6′-deoxychalcone.”FEBS Lett. 236:222-225, (1988).
International Search Report from International Application PCT/US00/20674.
Gilliland and Ladner, Crystallization of biological macromolecules for X-ray diffraction studies. Curr. Opin. Struct. Biol. 6:595-603, 1996.
Ke and Doudna, Crystallization of RNA and RNA-protein complexes. Methods, 34:408-414, 2004.
Wiencek, New strategies for protein crystal growth, Ann. Rev. Biomed. Eng., 1:505-534, 1999.
Cohen, N. C. et al., “Molecular Modeling Software and Methods for Medicinal Chemistry”,J Med. Chem., 33:883-894 (1990).
Tropf et al., Evidence that stilbene synthases have developed from chalcone synthases several times in the course of evolution. J Mol Evol Jun. 1994 38(6):610-8.
Lukacin et al., Transformation of acridone synthase to chalcone synthase. FEBS Letters (2001), p. 413-417.
Cohen et al., Molecular modeling software and methods for medicinal chemistry, J. Med. Chem., 1990, 33:883-894.
Gilliland and Ladner, Crystallization of biological macromolecules for X-ray diffraction studies, Curr.Opin. Struct. Biol., 6:595-603, 1996.
Interview Summary dated Feb. 2, 2007 for U.S. Appl. No. 10/450,230.
Ke and Doudna, Crystallization of RNA and RNA-protein complexes, Methods, 34:408-414, 2004.
Lukacin et al, Transformation of acridone synthase to chalcone synthase, FEBS Lett., (2001), 413-417.
Notice of Allowance dated Jun. 19, 2007 for U.S. Appl. No. 10/450,230.
Notice of Allowance dated Dec. 21, 2006 for U.S. Appl. No. 10/031,918.
Office Action dated May 25, 2005 for U.S. Appl. No. 10/031,918.
Office Action dated May 31, 2006 for U.S. Appl. No. 10/031,918.
Office Action dated Jun. 23, 2006 for U.S. Appl. No. 10/450,230.
Office Action dated Oct. 26, 2006 for U.S. Appl. No. 10/450,230.
Tropf et al., Evidence that stilbene synthase have developed from chalcone synthase several time in the course of evolution, J. Mol. Evol., (1994) 38:610-618.
Austin Michael B.
Bowman Marianne E.
Noel Joseph P.
Foley & Lardner LLP
Nashed Nashaat T
Reiter Stephen E.
The Salk Institute for Biological Studies
LandOfFree
Methods of producing polyketide synthase mutants and... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods of producing polyketide synthase mutants and..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods of producing polyketide synthase mutants and... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4214229