Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Patent
1997-02-18
1998-12-08
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
435111, 435115, 4352521, 43525232, 435840, 435843, C12P 1308, C12P 1314, C12P 121, C12P 1509
Patent
active
058467904
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL FIELD
The present invention relates to methods of producing L-lysine and L-glutamic acid by fermentation. L-lysine is widely used as a feed additive, etc., and L-glutamic acid is widely used as a material for seasonings, etc.
BACKGROUND ART
L-lysine and L-glutamic acid have been hitherto industrially produced by fermentatative methods by using coryneform bacteria belonging to the genus Brevibacterium or Corynebacterium having abilities to produce these amino acids. In these methods, it is known that the coryneform bacteria require biotin for their growth, while L-glutamic acid is not accumulated if an excessive amount of biotin exists in a medium. Therefore, any one of the following methods has been adopted in the conventional method of producing L-glutamic acid. Namely, cultivation is conducted in a medium in which the concentration of biotin is restricted, or cultivation is conducted such that a surfactant or a lactam antibiotic as a biotin action-suppressing agent is allowed to be contained in a medium at an initial or intermediate stage of cultivation in the case of use of the medium containing a sufficient amount of biotin.
However, especially when a material such as waste molasses, which is inexpensive but contains an excessive amount of biotin, is used as a carbon source in a medium, the biotin action-suppressing agent, which is required to be added to the medium, has been a cause to increase the production cost.
On the other hand, the following methods are known for simultaneous production of L-lysine and L-glutamic acid by fermentation. Namely, an L-lysine-producing bacterium is cultivated under a condition for L-glutamic acid production, or an L-lysine-producing bacterium and an L-glutamic acid-producing bacterium are mixed and cultivated (Japanese Patent Laid-open No. 5-3793).
However, in the method in which an L-lysine-producing bacterium is cultivated under a condition for L-glutamic acid production to simultaneously produce L-lysine and L-glutamic acid by fermentation, the condition for L-glutamic acid production is either that a biotin-auxotrophic bacterium belonging to the genus Brevibacterium or Corynebacterium is cultivated in a medium containing a low concentration of biotin, or that it is cultivated such that a surfactant or a lactam antibiotic is allowed to be contained in a medium at an initial or intermediate stage of cultivation in the case of the medium containing a sufficient amount of biotin. Especially when a material such as waste molasses, which is inexpensive but contains an excessive amount of biotin, is used as a carbon source in a medium, the surfactant or the lactam antibiotic as a biotin action-suppressing agent to be added to the medium has been a cause to increase the production cost.
Further, in the method in which an L-lysine-producing bacterium and an L-glutamic acid-producing bacterium are mixed and cultivated, there has been a problem that control of cultivation is difficult, and fermentation results are unstable.
DISCLOSURE OF THE INVENTION
An object of the present invention is to provide a method of producing L-glutamic acid inexpensively and stably by fermentation in which no biotin action-suppressing agent is added even when a material such as waste molasses containing an excessive amount of biotin is used as a carbon source in a medium.
Another object of the present invention is to provide a method of simultaneously producing L-lysine and L-glutamic acid inexpensively and stably by fermentation in which no biotin action-suppressing agent is added even when a material such as waste molasses containing an excessive amount of biotin is used as a carbon source in a medium.
As a result of vigorous investigations in order to achieve the aforementioned objects, the present inventors have found that a mutant strain obtained by giving temperature sensitivity to a biotin action-suppressing agent to a conventionally used coryneform L-glutamic acid-producing bacterium produces and accumulates a considerable amount of L-glutamic acid even in a medium cont
REFERENCES:
patent: 3971701 (1976-07-01), Takinami et al.
patent: 4334020 (1982-06-01), Nakazawa et al.
Asakura Yoko
Inoue Sumio
Kawahara Yoshio
Kimura Eiichiro
Nakamatsu Tsuyoshi
Ajinomoto Co. Inc.
Lilling Herbert J.
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