Methods of producing HBsAg

Details Methods of producing HBsAg Methods of producing HBsAg

1981-01-16

1983-11-22

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435240, 435241, C12N 500, C12N 502, C12P 2100

Patent

active

044169868

ABSTRACT:
Hepatitis B surface antigen (HBsAg) is produced in vitro in high titer and purity from tissue cultures of cells that shed HBsAg. The cells are grown on hollow fiber capillary units having a molecular weight cut-off of about 10,000.

REFERENCES:
patent: 3883393 (1975-05-01), Knazek et al.
patent: 4220725 (1980-09-01), Knazek et al.
patent: 4301250 (1981-11-01), McAleer
MacNab et al., Br. J. Cancer, 34, 509-515 (1976).
Kanzek et al., Science, 178, Oct. 6, 1972, pp. 65-66.
Quarles et al., In Vitro, 16 (2): 113-118, 1980.
Rutzky et al., JNCI vol. 63, No. 4, Oct. 1979, pp. 893-902.

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