Methods of producing and sequencing modified polynucleotides

Details Methods of producing and sequencing modified polynucleotides Methods of producing and sequencing modified polynucleotides

2009-12-07

2011-11-15

Riley, Jezia (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120, C435S091200, C536S023100, C536S024300, C536S024330

Reexamination Certificate

active

08058030

ABSTRACT:
The present invention encompasses methods for producing a modified polynucleotide sequence that comprises a (e.g., one or more) phosphorothiolate linkage, methods for determining a polynucleotide sequence comprising a (e.g., one or more) phosphorothiolate linkage, and methods for separating forward and reverse extension products that comprise a (e.g., one or more) phosphorothiolate linkage. The invention also encompasses kits for producing and/or determining the sequence of a modified polynucleotide that comprises a (e.g., one or more) phosphorothiolate linkage.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5665572 (1997-09-01), Ikeda et al.
patent: 5705628 (1998-01-01), Hawkins
patent: 5898071 (1999-04-01), Hawkins
patent: 5939292 (1999-08-01), Gelfand et al.
patent: 6087095 (2000-07-01), Rosenthal et al.
patent: 6117634 (2000-09-01), Langmore et al.
patent: 6329178 (2001-12-01), Patel et al.
patent: 6406848 (2002-06-01), Bridgham et al.
patent: 6500650 (2002-12-01), Stanton et al.
patent: 6534262 (2003-03-01), McKernan et al.
patent: 6537757 (2003-03-01), Langmore et al.
patent: 6828100 (2004-12-01), Ronaghi
patent: 6858393 (2005-02-01), Anderson et al.
patent: 6887690 (2005-05-01), Fisher et al.
patent: 7645866 (2010-01-01), Smith et al.
patent: 2002/0106686 (2002-08-01), McKernan
patent: 2010/0297628 (2010-11-01), Smith et al.
patent: 2010/0298551 (2010-11-01), Smith et al.
patent: 2002525129 (2002-08-01), None
patent: WO-93/21340 (1993-10-01), None
patent: WO-98/14610 (1998-09-01), None
patent: WO-98/39485 (1998-09-01), None
patent: WO-00/18967 (2000-04-01), None
patent: WO-03/076037 (2003-09-01), None
patent: WO-2007/002890 (2007-01-01), None
EP06785931.4 Office Action mailed on Jun. 3, 2009.
EP06785931.4 Response to Jun. 3, 2009 Office action filed Oct. 13, 2009.
EP06785931.4 Search Report mailed Jun. 3, 2009.
U.S. Appl. No. 11/476,423 Nonfinal Office Action mailed Feb. 20, 2009.
U.S. Appl. No. 11/476,423 Nonfinal Office Action Response filed Aug. 26, 2009.
U.S. Appl. No. 12/632,740 Non Final Office Action mailed Oct. 27, 2010.
U.S. Appl. No. 12/632,740, Nonfinal Office Action Response filed Jan. 27, 2011.
JP2008-519600 Office Action mailed Dec. 10, 2010.
Das, M et al., Full-length cDNAs: more than just reaching the ends, Physiol Genomics vol. 6, pp. 57-80 (2001).
Lin, T et al., 3′-5′ Exonucleolytic Activity of DNA Polymerases: Structural Features That Allow kinetic Discrimination between Ribo- and Deoxyribonucleotide Residues, Biochemistry, vol. 40, pp. 8749-8755 (2001).
Mag, M et al., Synthesis and selective cleavage of an oligodeoxynucleotide containing a bridged internucleotide 5′-phosphorothioate linkage, Nuc Acids Res, vol. 19(7), pp. 1437-1441 (1991).
Maxam, A. et al., A new method for sequencing DNA, PNAS, vol. 74, pp. 560-564 (1977).
PCT/US06/025529 International Search Report mailed on Jul. 5, 2007.
PCT/US06/025529 International Written Opinion issued Jan. 9, 2008.
PCT/US06/025529 International Preliminary Report on Patentability issued Jan. 9, 2008, pp. 1-11.
Sanger, F. et al., DNA sequencing with chain-terminating inhibitors PNAS, vol. 74(12), pp. 5463-5467 (1977).
Sauer, S. et al., A novel procedure for efficient genotyping of single nucleotide polymorphisms, Nuc Acids Res, vol. 28(5), E13, (2000).
STRATAGENE Catalog, Gene Characterization Kits(1998).
Vyle, J. S. et al., Sequence- and strand-specific cleavage in oligodeoxyribonucleotides and DNA containing 3′-thiothymidine, Biochemistry, vol. 31(11), pp. 3012-3018 (1992).
Xia, G et al., “Directed evolution of novel polymerase activities: Mutation of a DNA polymerase into an efficient RNA polymerase”, PNAS, vol. 99(10), pp. 6597-6602 (2002).
EP06785931.4 Office action mailed Feb. 17, 2011.
U.S. Appl. No. 12/632,730 Office action mailed Mar. 3, 2011.
Sontheimer, E., “Bridging Sulfur Substitutions in the Analysis of Pre-mRNA Splicing”,Methods: A Companion to Methods in Enzymology,vol. 18, 1999, pp. 29-37.
Yuzhakov, A., “3′-Mercapto-2′,3′-dideoxynucleotides are high effective terminators of DNA synthesis catalyzed by HIV reverse transcriptase”,FEBS Letters;vol. 306 (2,3), 1992, pp. 185-188.

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