Methods of preparing tissue plasminogen activator derivatives

Details Methods of preparing tissue plasminogen activator derivatives Methods of preparing tissue plasminogen activator derivatives

1994-03-17

1998-03-17

Jacobson, Dian C.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

424 9464, 435212, 4352523, 435359, C12N 121, C12N 510, C12N 964, C12N 1555

Patent

active

057285659

ABSTRACT:
Tissue plasminogen activator (t-PA) derivatives are produced in useful quantities using recombinant DNA techniques. Specific derivatives include amino acid deletion derivatives and amino acid substitution derivatives. A deletion derivative lacking the N-terminal first 68 amino acids is specifically exemplified having requisite t-PA characteristics. The invention disclosed thus enables the production of t-PA derivatives via recombinant means. Methods, expression vehicles and various host cells useful in the production of said t-PA derivatives are also disclosed.

REFERENCES:
patent: 3904480 (1975-09-01), Hull et al.
patent: 3998947 (1976-12-01), D'Hinterland et al.
patent: 4245051 (1981-01-01), Reich et al.
patent: 4259447 (1981-03-01), Hafeli
patent: 4314994 (1982-02-01), d'Hinterland et al.
patent: 4317882 (1982-03-01), Horiguchi et al.
patent: 4370417 (1983-01-01), Hung et al.
patent: 4505893 (1985-03-01), Muri et al.
patent: 4752603 (1988-06-01), Collen
patent: 4766075 (1988-08-01), Goeddel et al.
patent: 4853330 (1989-08-01), Goeddel et al.
patent: 5011795 (1991-04-01), Levinson
patent: 5094953 (1992-03-01), Anderson
patent: 5185259 (1993-02-01), Goeddel et al.
Camiolo et al., (1971), PSEBM, 138:277.
Thorsen et al., (1972), Thrombos, Diathes, Haemorrh. 28.65.
Granelli-Piperno et al., (1978), J. Exp. Med. 148:223.
Thorsen (1977), Danish Med. Bulletin, 24:189.
Kingsman et al., Genetic Engineering, Blackwell.
Scientific Publications, pp. 110-121 (1988.
Wallen (1978), Progress in Chem. Fibrinolysis and Thrombolysis, 3:167.
Wilson et al., (1980), Chem. Ab., 92, No. 144679a.
Collen (1980), Edward Kowalski Memorial Lecture Thromb. Haem., 43:77.
Wun et al., (1982), Jrnl. Biol. Chem., 257:3276.
Ninth Collected Index of Chem. Abstr.
Rijken et al., (1980), Thromb. Res., 18:815.
Wigler et al., (1980), Proc. Natl. Acad. Sci. U.S.A. 77:3567.
Kaufman et al., (1982), J. Mol. Biol., 159:601.
Kaufman et al., (1982), Gene Applification, pp. 245-250.
Weissmann, et al., (1982), Phil. Trans. R. Soc. Lond. 299:7.
The Newswatch, (1982), McGraw-Hill's Biotech. Newswatch 2:1.
Kaufman et al., (1982), Molecul. Cell. Biol., 2:1304.
Rougeon et al., (1975), Nucl. Acids Res., 2:2365.
Rabbitts (1976), Nature, 260:221.
Maniatis et al., (1976), Cell, 8:163.
O'Malley et al., (1976), ICN-UCLA Symposium, 5:309.
Salser et al., (1976), Prog. Nucl. Acids Res. Mol. Biol., 19:177.
Higuchi et al., (1976), Proc. Nat. Acad. Sci. U.S.A. 73:3146.
McReynolds et al., (1977), J. Biol. Chem., 252:1840.
Rougeon et al., (1977), Gene, 1:229.
Monahan et al., (1977), J. Biol. Chem., 252:4722.
Liu et al., (1977), Science, 196:192.
Humphrfes et al., (9177), Nucl. Acids Res., 4:2389.
Ullrich et al., (1977), Science, 196:1313.
McReynolds et al., (1977), Gene, 2:217.
Seeburg et al., (9177), Nature, 270:486.
Shine et al., (1977), Nature, 270:494.
Seidman et al., (1978), Nature, 271:582.
Wilson et al., (1978), Nucl. Acids Res., 5:563.
Little et al., (1978), Nature, 273:640.
Cummings et al., (1978), Nature, 276:418.
Harpold et al., (1978), Nucl. Acids Res., 5:2039.
Sim et al., (1978), Cold Spring Harbor Symp. Quant. Biol. 42:933.
Villa-Komaroff, (1978), Proc. Nat. Acad. Sci. U.S.A., 75:3727.
Gordon et al., (1978), J. Biol. Chem., 253:8629.
Wall et al., (1978), Nucl. Acids Res. 5:3113.
Sippel et al., (1978), Nucl. Acids Res., 5:3275.
Wahli et al., (1978), Dev. Biol., 67:371.
Lehrach et al., (1978), Proc. Nat. Acad. Sci. U.S.A., 75:5417.
Schibler et al., (1978), Cell, 15:1495.
Sobel et al., (1978), Proc. Nat. Acad. Sci. U.S.A., 75:5846.
Chang et al., (1978), Nature, 275:617.
Poon et al., (1978), Nucl. Acids Res., 5:4625.
Nakanishi et al., (1978), Proc. Nat. Acad. Sci. U.S.A., 75:6021.
Yamamoto et al., (1980), J. Biol. Chem., 255:2612.
Early et al., (1979), Proc. Nat. Acad. Sci. U.S.A., 76:857.
Innis et al., (1979), Arch. Biochem. Biophys., 195:128.
Gubbins et al., (1979), Nucl. Acids Res., 6:915.
King et al., (1979), J. Biol. Chem., 254:6781.
Roberts et al., (1979), Proc. Nat. Acad. Sci. U.S.A., 76:2153.
Lehrach et al., (1979), Biochemistry, 18:3146.
Rogers et al., (1979), Nucl. Acids Res., 6:3305.
Tilghman et al., (1979), J. Biol. Chem., 254:7393.
Cochet et al., (1979), Nucl. Acids Res., 6:2435.
Buelll et al., (1979), J. Biol. Chem., 254:9277.
Wahl et al., (1979), J. Biol. Chem., 254:8679.
Sargent et al., (1979), Proc. Natl. Acad. Sci., U.S.A., 76:3256.
Bhat et al., (1979), Proc. Nat. Acad. Sci. U.S.A., 76:3299.
Fantoni et al., (1979), Nucl. Acids Res., 6:3505.
Martial et al., (1979), Science, 205:602.
Kioussis et al., (1979), Proc. Nat. Acad. Sci., 76:4370.
Ohno et al., (1980), Biochem. Biophys. Acta., 606:34.
Fiddes et al., (1979), Nature, 281:351.
Goeddel et al., (1979), Nature, 281-544.
Kronenberg et al (1979), Proc. Nat. Acad. Sci., 76:4981.
Roskam et al., (1979), Nucl. Acids Res., 7:305.
Liebscher et al., (1980), Gene, 9:233.
Chan et al., (1979),, Proc. Nat. Acad. Sci. U.S.A., 76:5036.
Bell et al., (1979), Nature, 282:525.
Sim et al., (1979), Cell, 18:1303.
Tucker et al., (1979), Science, 206:1299.
Richards et al., (1979), Nucl. Acids Res., 7:1137.
Gough et al., (1980), Proc. Nat. Acad. Sci. U.S.A., 77:554.
Firtel et al., (1979), Proc. Nat. Acad. Sci. U.S.A., 76:6206.
Adams et al., (1980), Biochemistry, 19:2711.
Gough et al., (1980), Biochemistry, 19:2702.
Obata et al., (1980), Gene, 9:87.
Katcoff et al., (1980), Proc. Nat. Acad. Sci. U.S.A.77:960.
Joho et al., (1980), Proc. Nat. Acad. Sci. U.S.A.77:1106.
Wieringa et al., (1979), Nucl. Acids Res., 7:2147.
Merlino et al., (1980), Proc. Nat. Acad. Sci. U.S.A., 77:765.
Calame et al., (1980), Nature, 284:452.
Taniguchi et al., (1979), Proc. Japan Acad.55:464.
Hoeijmakers et al., (1980), Gene, 8:391.
Law et al., (1980), Gene, 10:53.
Meyuhas et al., (1980), Gene, 10:113.
Griffin-Shea et al., (1980), Cell, 19:915.
Cleveland et al., (1980), Cell, 20:95.
Matthyssens et al., (1980), Nucl. Acids Res., 8:703.
Nagata et al., (1980), Nature, 284:316.
Cooke et al., (1981), J. Biol. Chem., 256:4007.
Cooke et al., (1980), J. Biol. Chem., 255:6502.
Dunnick et al., (1980), Nucl. Acids Res., 8:1475.
Vamvakopoulos et al., (1980), Proc. Nat. Acad. Sci., U.S.A., 77:3149.
Auffray et al., (1980), Nucl. Acids Res., 8:1231.
Deacon et al., (1980), Nucl. Acids Res., 8:1187.
Norgard et al., (1980), J. Biol. Chem., 255:7665.
Nilson et al., (1980), Nucl. Acids Res., 8:1561.
Tsitilou et al., (1980), Nucl. Acids Res. 8:1987.
Gorecki et al., (1980), Proc. Nat. Acad. Sci. U.S.A.77:3686.
Hobart et al., (1980), Nature, 288:137.
Sures et al., (1980), Science, 208:57.
Derynck et al., (1980), Nature, 285:542.
Fiddes et al., (1980), Nature, 286:684.
Richards et al., (1980), J. Biol. Chem., 255:9306.
Schwartz et al., (1980), Biochem.19:5883.
Schibler et al., (1980), J. Mol. Biol., 142:93.
Liao et al., (1980), J. Biol. Chem.255:8046.
Taniquchi et al., (1980), Proc. Nat. Acad. Sci. U.S.A., 77:4003.
Ordahl et al., (1980), Proc. Nat. Acad. Sci. U.S.A., 77:4519.
Amara et al., (1980), Proc. Nat. Acad. Sci. U.S.A., 77:4444.
Miller et al., (1980), J. Biol. Chem., 255:7521.
Chandra et al., (1980), Biochem. Biophys. Res. Commun., 95:197.
Christophe et al., (1980), Eur. J. Biochem.111:419.
Zakut et al., (1980), Nucl. Acids. Res., 8:3591.
Hobart et al., (1980), Science, 210:1360.
MacGillivray, (1980), Proc. Nat. Acad. Sci. U.S.A., 77:5153.
Dolby et al., (1980), Proc. Nat. Acad. Sci. U.S.A., 77:6027.
MacDonald et al., (1980), Nature, 287:117.
Fagan et al., (1981), J. Biol. Chem., 256:520.
Streuli et al., (1980), Science, 209:1343.
Ginzburg et al., (1980), Nucl. Acids Res., 8:3553.
Cozens et al., (1980), Eur. J. Biochem., 112:443.
Goeddel et al., (1980), Nature, 287:411.
Medford et al., (1980), Proc. Nat. Acad. Sci. U.S.A., 77:5749.
Richards et al., (1981), J. Biol. Chem.256:526.
Odink et al., (1981), J. Biol. Chem., 256:1453.
Bedbrook et al., (1980), Nature, 287:692.
Miller et al., (1980), Endocrinology, 107:851.
Goodman et al., (1980), Proc. Nat. Acad. Sci. U.S.A.77:5869.
Sun et al., (1981), Nature, 289:37.
Auffray et al., (1980), Gene, 12:77.
Minty et al., (1981), J. Biol. Chem., 256:1008.
Ploegh et al., (1980), Proc. Nat. Acad. Sci.

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