Methods of PHA extraction and recovery using non-halogenated sol

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

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203 14, 203 67, 210767, 210768, 210787, 435134, 435146, 528354, 528361, 528491, 528492, 528501, C12P 742, C12P 762, C12N 120, C08G 6306

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06043063&

ABSTRACT:
Polyhydroxyalkanoate (PHA) polyester is extracted from biomass by dissolving the PHA in a non-halogenated solvent which comprises a PHA-good solvent or a mixture thereof. Suitable PHA-good solvents can be selected from the disclosed alcohols, esters, amides and ketones. The PHA can be recovered, for example, by cooling, by solvent evaporation, or by addition of a PHA-poor solvent, wherein the PHA-poor solvent preferably dissolves less than about 1% (w/v) of the PHA at a temperature below the solvent boiling point. Preferred PHA types for use in the invention are poly (hydroxybutyrate-co-hydroxyvalerate), poly(3-hydroxybutyrate-co-4-hydroxybutyrate), and polymers/copolymers of hydroxyterminated polyhydroxybutyrate.

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patent: 5821299 (1998-10-01), Noda

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