Methods of performing gene trapping in bacterial and bacteriopha

Details Methods of performing gene trapping in bacterial and bacteriopha Methods of performing gene trapping in bacterial and bacteriopha

1998-06-22

2000-12-05

Yucel, Remy

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

Introduction of a polynucleotide molecule into or...

4353201, 435455, 435462, 435463, 435473, C12N 1587

Patent

active

061565741

ABSTRACT:
A method of efficiently sequencing multiple exons from complex genomic DNAs is disclosed. The methodology includes the use of bacterial and bacteriophage-derived artificial chromosomes (BBPACs) in novel gene trapping protocols. Targeted gene trapping by homologous recombination, and random gene trapping with the use of a transposon system are exemplified. Included in the invention are methods of preparing a gene map from BBPAC contigs, the resulting gene maps, methods of constructing a cDNA library from BBPAC contigs, and the resulting cDNA libraries.

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