Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-01-13
2010-11-16
Salimi, Ali R. (Department: 1648)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
Reexamination Certificate
active
07833707
ABSTRACT:
An improved method for recovering the protein expressed by open reading frame 2 from PCV2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.
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Molecular
Eichmeyer Marc
Nitzel Greg
Schaeffer Merrill
Boehringer Ingelheim Vetmedica, Inc.
Morris Michael P.
Salimi Ali R.
Scott Julie A.
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