Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Patent
1996-04-04
1999-08-24
Fox, David T.
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
435268, 435419, 536 232, 536 237, 800281, A01H 500, C12N 514, C12N 1531, C12N 1552
Patent
active
059426608
ABSTRACT:
Genes and methods for optimizing levels of substrates employed in the biosynthesis of copolymers of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) in plants and bacteria via manipulation of normal metabolic pathways using recombinant DNA techniques are provided. This is achieved through the use of a variety of wild-type and/or deregulated enzymes involved in the biosynthesis of aspartate family amino acids, and wild-type or deregulated forms of enzymes, such as threonine deaminase, involved in the conversion of threonine to P(3HB-co-3HV) copolymer end product. By these methods, enhanced levels of threonine, .alpha.-ketobutyrate, propionate, propionyl-CoA, .beta.-ketovaleryl-CoA, and .beta.-hydroxyvaleryl-CoA are produced. Also provided are methods for the biological production of P(3HB-co-3HV) copolymers in plants and bacteria utilizing propionyl-CoA produced through a variety of engineered metabolic pathways. Introduction into plants and bacteria of an appropriate .beta.-ketothiolase, .beta.-ketoacyl-CoA reductase, and PHA synthase, alone or in combination with various enzymes involved in asparate family amino acid biosynthesis and the conversion of threonine to PHA copolymer precursors, will permit these organisms to produce P(3HB-co-3HV) copolymers.
REFERENCES:
patent: 5416011 (1995-05-01), Hinchee et al.
patent: 5502273 (1996-03-01), Bright et al.
Priefert et al., (1992) "Identification and Molecular Characterization of the Acetyl Coenzyme A Synthetase Gene (acoE) of Alcaligenes eurtrophus," Journal of Bacteriology, 174:6590-6599.
Steinbuchel, (1991), "Polyhydroxyalkanoic Acids," Biomaterials, Stockton Press, New York, 125-213.
Fry et al., (1987) "Transformation of Brassica napus with Agrobacterium tumefaciens based vectors," Plant Cell Reports, 6:321-325.
Mourad et al., (1995) "L-O-Methylthreonine-Resistant Mutant of Arabidopisis Defective in Isoleucine Feedback Regulation," Plant Physiology, 107:43-52.
Taillon et al., (1988) "Analysis of the functional domains of biosynthetic threonine deaminase by comparison of the amino acid sequences of three wild-type alleles to the amino acid sequence of biodegradative threonine daminase," Gene., 63:245-252.
Bisswanger, (1981), "Substrate Specificity of the Pyruvate Dehydrogenase Complex from Escherichia coli," Journal of Biological Chemistry, 256:815-822.
LaRossa RA, et al. Toxic accumulation of alpha-ketobutyrate caused by inhibition of the branched-chain amino acid biosynthetic enzyme acetolactate synthase in Salmonella typhimurium. JBC 169: 1372-1378, Apr. 1987.
Taillon BE., et al. "Analysis of the functional domains of biosynthetic threonine deaminase by comparison of the amino acid sequences of three wild-type alleles to the amino acid sequence of biodegradative threonine deaminase." Gene 3: 245-252, 1988.
Lawther RP, et al. The complete nucleotide sequence of the ilvGMEDA operon of Escheria coli K-12. Nucl. Acids Res. 15: 2137-2155, 1987.
Nawrath et al., (1994) "Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation," Proceedings of National Academy of Science USA, 91:12760-12764.
Poirier et al., (1992) "Polyhydroxybutyrate, a Biodegradable Thermoplastic, Produced in Transgenic Plants," Science, 256:520-523.
Haywood et al., (1988) "Characterization of two 3-ketothiolases possessing differing substrate specificities in the polyhydroxyalkanoate synthesizing organism Alcaligenes eutrophus," Federation of European Microbiological Societies, 52:91-96.
Manchak et al., (1994) "Control of polyhydroxyalkanoate synthesis in Azotobacter vinelandii strain UWD," Microbiology, 140:953-963.
Rhie et al., (1995) "Role of fadR and atoC(Con) Mutations in Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) Synthesis in Recombinant pha.sup.+ Escherichia coli," Applied and Environmental Microbiology, 61:2487-2492.
Eisenstein et al., (1995) "An Expanded Two-State Model Accounts for Homotropic Cooperativity in Biosynthetic Threonine Deaminase from Escherichia coli," Biochemistry, 34:9403-9412.
Feldberg et al., (1971) "L-Threonine Deaminase of Rhodospirillum rubrum, Purification and Characterization," European Journal Biochemistry, 21:438-446.
Eisenstein, (1991) "Cloning, Expression, Purification, and Characterization of Biosynthetic Threonine Deaminase from Escherichia coli," Journal of Biological Chemistry, 266:5801-5807.
Nakamura et al., (1992) "Biosynthesis of poly(3-hydroxyalkanoate) from amino acids," International Journal of Biological Macromol., 14:321-325.
Galili, (1995) "Regulation of Lysine and Threonine Synthesis," The Plant Cell, 7:899-906.
Slater et al., (1988) "Cloning and Expression in Escherichia coli of the Alcaligenes eutrophus H16 Poly-.beta.-Hydroxybutyrate Biosynthetic Pathway," Journal of Bacteriology, 170:4431-4436.
Slater et al., (1992) "Production of Poly-(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) in a Recombinant Escherichia coli Strain," Applied and Environmental Microbiology, 58:1089-1094.
Schubert et al., (1988) "Cloning of the Alcaligenes eutrophus Genes for Synthesis of Poly-.beta.-Hydroxybutyric Acid (PHB) and Synthesis of PHB in Escherichia coli," Journal of Bacteriology, 170:5837-5847.
Peoples et al., (1989) "Poly-.beta.-hydroxybutyrate (PHB) Biosynthesis in Alcaligenes eutrophus H16," Journal of Biological Chemistry, 264:15293-15297.
Peoples et al., (1989) "Poly-.beta.-hydroxybutyrate Biosynthesis in Alcaligenes eutrophus H16," Journal of Biological Chemistry, 264:15298-15303.
Barwale et al., (1986) "Plant regeneration from callus cultures of several soybean genotypes via embryogenesis and organogenesis," Planta, 167:473-481.
Cakmak et al., (1989) "Effect of Zinc Nutritional Status on Growth, Protein Metabolism and Levels of Indole-3-acetic Acid and other Phytohormones in Bean (Phaseolus vulgaris L.)," Journal of Experimental Botany, 40:405-412.
Wright et al., (1986) "Plant regeneration by organogenesis in Glycine max," Plant Cell Reports, 5:150-154.
Barwale et al., (1986) "Screening of Glycine max and Glycine soja genotypes for multiple shoot formation at the cotyledonary node," Theoretical Applied Genetics, 72:423-438.
Wright et al., "Regeneration of soybean (Glycine max L. Merr.) from cultured primary leaf tissue," Plant Cell Reports, 6:83-89.
Kim et al., (1994) "Synergistic effects of proline and inorganic micronutrients and effects of individual micronutrients on soybean (Glycine max shoot regeneration in vitro," Journal Plant Physiology, 144:726-734.
Yang et al., (1990) "Comparitive studies of organogenesis and plant regeneration in various soybean explants," Plant Science, 72:101-108.
Chee et al., (1989) "Transformation of soybean (Glycine max) by infecting germinating seeds with Agrobacterium tumefaceins," Plant Physiology, 91:1212-1218.
Christou et al., (1992) "Prediction of germ-line transformation events in chimeric Ro transgenic soybean plantlets using tissue-specific expression patterns," The Plant Journal, 2:283-290.
Falco et al., (1995) "Transgenic canola and soybean seeds with increased lysine," Bio/Technology, 13:477-582.
Finer et al., (1991) "Transformation of soybean via particle bombardment of embryogenic suspension culture tissue," In Vitro Cell. Dev. Biol., 27P:175-182.
Hinchee et al., (1988) "Production of transgenic soybean plants using Agrobacterium-mediated DNA transfer," Bio/Technology, 6:915-922.
McCabe et al., (1988) "Stable transformation of soybean (Glycine max) by particle acceleration," Bio/Technology, 6:923-926.
Owens et al., (1985) "Genotypic variability of soybean response to Agrobacterium strains harboring the Ti or Ri plasmids," Plant Physiology, 77:87-94.
Parrott et al., (1994) "Recovery and evaluation of soybean plants transgenic for a Bacillus thuringiensis var. Kurstaki insecticidal gene," In Vitro Cell. Dev. Biology, 30P:144-149.
Padgette et al., (1995) "Development, identification and characterization of a glyphosate-tolerant soybean line," Crop Science, 35:1451-1461.
Cheng et al., (1980) "Plant regeneration from soybean cotyledonary node segments in culture," Plant Science Letters, 19:
Gruys Kenneth James
Kishore Ganesh Murthy
Mitsky Timothy Albert
Padgette Stephen Rogers
Slater Steven Charles
Bond Gary M.
Fox David T.
Monsanto Company
Nelson Amy J.
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