Methods of obtaining epothilone D using crystallization and...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C548S204000

Reexamination Certificate

active

06998256

ABSTRACT:
Methods are provided for purification of epothilone D and epothilone D analogs from epothilone producing cells such as, for example,Sorangium cellulosumandMyxococcus xanthus(recombinant). Epothilone can be purified from fermentation broth, for example by using resins, chromatography, and crystallization. Epothilone D can be crystallized from a binary solvent system in which water is the forcing solvent.

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