Methods of obtaining compositions enriched for hematopoietic ste

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 2, 435 30, 4352402, 43524027, 5303887, C07K 1628, C12N 508, C12Q 124

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056771363

ABSTRACT:
A method for obtaining human hematopoietic stem cells is provided by enrichment for stem cells using a novel stem cell marker. Compositions enriched for stem cells are obtained using novel antibody HCC-1 which is specific for a CD59 epitope highly accessible on stem cells, and less accessible or absent on mature cells. The enriched population of cells derived from these methods are also provided.

REFERENCES:
patent: 4714680 (1987-12-01), Civin
patent: 5061620 (1991-10-01), Tsukamoto et al.
patent: 5147784 (1992-09-01), Peault
patent: 5468612 (1995-11-01), Cohen et al.
Baum et al., "Isolation of a candidate human hematopoietic stem-cell population" Proc. Natl. Acad. Sci. USA (1992) 89:2804-2808.
Chaudhary et al., "Expression and activity of P-glycoprotein, a multidrug efflux pump, in human hematopoietic stem cells" Cell (1991) 66:85-94.
Spangrude et al.,"Purification and characterization of mouse hematopoietic stem cells" Science (1988) 241:58-62.
Smith et al., "Clonal analysis of hematopoietic stem-cell differentiation in vivo" Proc. Natl. Acad. Sci. USA (1991) 88:2788-2792.
Uchida, "Characterization of mouse hematopoietic stem cells (bone marrow cells)" Dialog.TM. Dissertation Abstact (1992) Ph.D. Thesis, Stanford University, 2 pages total.
Whitlock et al., "Long-term culture of B lymphocytes and their precursors from murine bone marrow" Proc. Natl. Acad. Sci. USA (1982) 79:3608-3612.
Whitlock et al., "Bone marrow stromal cell lines with lymphopoietic activity express high levels of a pre-B neoplasia-associated molecule" Cell (1987) 48:1009-1021.
Stefanova et al., "Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules" J.Immunol. (1991) 147:1587-1592.
Spangrude,"Enrichment of murine haemopoietic stem cells: diverging roads" Immunol. Today (1989) 10:344-350.
Sawada et al., "Complementary DNA sequence and deduced peptide sequence for CD59/MEM-43 antigen, the human homologue of murine lymphocyte antigen Ly-6C" Nucl. Acids Res. (1989) 17:6728.
Philbrick et al., "The CD59 antigen is a structural homologue of murine Ly-6 antigens but lacks interferon inducibility" Eur. J. Immunol. (1990) 20:87-92.
Davies et al., "CD59, an Ly-6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells" J. Exp. Med. (1989) 170:637-654.
Okada et al., "20 KDa homologous restriction factor of complement resembles T cell activating protein" Biochem. Biophys. Res. Commun. (1989) 162:1553-1559.
Haugland, Handbook of Fluorescent Probes and Research Chemicals Molecular Probes, Inc., 5th ed., (1992-1994). The title page and table of contents are included herewith.
Kyoizumi et al., "Implantation and maintenance of functional human bone marrow in SCID-hu mice" Blood (1992) 79:1704-1711.
Chen et al., "Engraftment of human hematopoietic precursor cells with secondary transfer potential in SCID-hu mice" Blood (1994) 84:2497-2505.
Kohler et al., "Continuous cultures of fused cells secreting antibody of predefined specificity" Nature (1975) 256:495-497.
Simmons et al., "Mechanisms of cytomegalovirus-mediated myelosuppression: Perturbation of stromal cell function versus direct infection of myeloid cells" Proc. Natl. Acad. Sci. USA (1990) 87:1386-1390.
Simmons et al.,"Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1" Blood (1991) 78:55-62.
Simmons et al., "Host origin of marrow stromal cells following allogeneic bone marrow transplantation" Nature (1987) 328:429-432.
Iscove et al., "Net increase of pluripotential hemapoietic precursors in suspension culture in response to IL-1 and IL-3" J. Immunol. (1989) 142:2332-2337.
Haylock et al., "Ex vivo expansion and maturation of peripheral blood CD34.sup.+ cells into the myeloid lineage" Blood (1992) 80:1405-1412.
To et al., "The optimization of collection of peripheral blood stem cells for autotransplantation in acute myeloid leukaemia" Bone Marrow Transplant. (1989) 4:41-47.
Sutherland et al., "Characterization and partial purification of human marrow cells capable of initiating long-term hematopoiesis in vitro" Blood (1989) 74:1563-1570.
Smith et al., "Purification and partial characterization of a human hematopoietic precursor population" Blood (1991) 77:2122-2128.
Brandt et al., "Cytokine-dependent long-term culture of highly enriched precursors of hematopoietic progenitor cells from human bone marrow" J. Clin. Invest. (1990) 86:932-941.
Andrews et al., "Precursors of colony-forming cells in humans can be distinguished from colony-forming cells by expression of the CD33 and CD34 antigens and light scatter properties" J. Exp. Med. (1989) 169:1721-1731.
Terstappen et al., "Sequential generations of hematopoietic colonies derived from single nonlineage-committed CD34.sup.+ CD38.sup.- progenitor cells" Blood (1991) 77:1218-1227.
Udomsakdi et al., "Separation of functionally distinct subpopulations of primitive human hematopoietic cells using rhodamine-123" Exp. Hematol. (1991) 19:338-342.
Rayner et al., "A simple and efficient procedure for generating stable expression libraries by cDNA cloning in a retroviral vector" Mol. Cell. Biol. (1994) 14:880-887.
Galy et al.,"Generation of T cells from cytokine-mobilized peripheral blood and adult bone marrow CD34.sup.+ cells" Blood (1994) 84:104-110.
Galy et al., "Precursors of CD.sup.+ CD4.sup.+ CD8.sup.+ cells in the human thymus are defined by expression of CD34. Delineation of early events in human thymic development" J. Exp. Med. (1993) 178:391-401.
L.W.M.M. Terstappen et al, Journal of Leukocyte Biology, 52, 652-660,1992.

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