Methods of obtaining a DNA sequence for integration site indepen

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 691, 435 911, 435 914, 4351723, 800 2, 800DIG1, 800DIG2, 536 241, C12N 1510, C12N 1585, C12N 1563, C12N 516

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056353553

ABSTRACT:
Vectors for the integration of a gene into the genetic material of a mammalian host cell such that the gene may be expressed by the host cell comprise a promoter and the gene and include a dominant activator sequence capable of eliciting host cell-type restricted, integration site independent, copy number dependent expression of the gene.

REFERENCES:
Chao et al., "The Regulated Expression of .beta.-Globin Genes Introduced into Mouse Erythroleukemia Cells", Cell, vol. 32, pp. 483-493, (1983).
Gross et al., "Nuclease Hypersensitive Sites in Chromatin", Ann. Rev. Biochem., vol. 57, pp. 159-197, (1988).
Grosschedl et al., "Introduction of a .mu. Immunoglobulin Gene into the Mouse Germ Line: Specific Expression in Lymphoid Cells and Synthesis of Function Antibody", Cell, vol. 38, pp. 647-658 (Oct. 1984).
Kollias et al., "Regulated Expression of Human .sup.A .gamma., .beta.-, and Hybrid .gamma..beta.-Globin Genes in Transgenic Mice: Manipulation of the Developmental Expression Patterns", Cell, vol. 46, pp. 89-94, (Jul. 4, 1986).
Lang et al., "The structure of the Human CD2 gene and its expression in transgenic mice", The EMBO Journal, vol. 7, No. 6, pp. 1675-1682, (Jun., 1988).
Li et al., "Nucleotide Sequence of 16-Kilobase Pairs of DNA 5 ' to the Human .epsilon.-Globin", The Journal of Biology and Chemistry, pp. 14901-14910.
Magram et al., "Developmental regulation of a cloned adult .beta.-globin gene in transgenic mice", Nature, vol. 315, pp. 338-340, (May, 1995).
Marks et al., "Erythroleukemic Differentiation", Ann. Rev. Biochem., vol. 47, pp. 419-448, (1978).
Tuan et al., "The .beta.-like-globin gene domain in human erythroid cells", Proc. Natl. Acad. Sci. USA, vol. 82, pp. 6384-6388, (Oct. 1985).

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