Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Reexamination Certificate
2005-04-26
2005-04-26
Winkler, Ulrike (Department: 1648)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
C424S093200, C424S093600, C424S208100, C435S006120, C435S008000, C435S014000, C435S034000, C435S070100, C435S173300, C435S207000, C435S242000, C435S320100, C435S372300, C435S455000, C514S04400A, C536S022100, C536S023400, C536S023720, C536S024100
Reexamination Certificate
active
06884576
ABSTRACT:
A method is provided for detecting a presence of HIV virus in a sample comprising: taking a culture of recombinant cells which (a) are capable of cell division, (b) express CD4 receptor and one or more additional cell surface receptors necessary to allow the HIV virus to infect, (c) enable the HIV virus to replicate and infect the noninfected cells in the cell culture, and (d) comprise a reporter sequence introduced into the recombinant cells comprising a reporter gene whose expression is regulated by a protein specific to HIV viruses which is expressed from a genome of an HIV virus upon infection of the recombinant cell by the HIV virus; contacting the cell culture with a sample to be analyzed for the presence of HIV virus in the sample; and detecting a change in a level of expression of the reporter gene in cells in the recombinant cell culture. The method can be used to detect the presence of HIV virus in a sample, detect the presence of different strains of HIV virus in a sample, detect HIV drug resistance in a sample, determine what combination of one or more anti-HIV agents would be effective in treating a patient, and screen compositions for anti-HIV activity.
REFERENCES:
patent: 5837464 (1998-11-01), Capon et al.
patent: 0123456 (2000-01-01), None
patent: 2 244 712 (1991-12-01), None
patent: WO 9305185 (1993-03-01), None
Jamieson, B.D. et al., “In Vivo Pathogensis of a Human Immunodeficiency Virus Type 1 Reporter Virus”, Journal of Virology, Aug. 1998, vol. 72, No. 8, pp. 6520-6526.
Dorsky, D.I. et al., “Detection of HIV-1 Infection With a Green Fluorescent Protein Reporter System”, Journal of Immune Deficiency Syndromes, vol. 13, No. 4, 1996.
Lee, A.H. et al., “Generation of the Replication-Competent Human Immunodeficiency Virus Type 1 Which Expresses A Jellyfish Green Fluorescent Protein”, Biochemical and Biophysical Research Communications. vol. 233, pp. 288-292, 1997.
Koseki, S. et al., A Simple Assay System For Examination of the Inhibitory Potential In Vivo of Decoy RNA's, Ribozymes and other Drugs By Measuring the Tat-Mediated Transcription of a Fusion Gene Composed of the Long Terminal Repeat of HIV-1 and a Gene For Luciferase.
Gervaix, A. et al., “A New Reporter Cell Line To Monitor HIV Infection and Drug Susceptibilty In Vitro”, Proc. Natl. Acad. Sci USA, vol. 94, pp. 4653-4658, Apr. 1997.
Vodicka et al., “Indicator cell lines for detection of primary strains of human and simian immunodeficiency viruses”, Virology. vol. 233, 1997, pp. 193-198.
Deng et al., “Identification of a major co-receptor for primary isolates of HIV-1”, Nature, vol. 381, 1996, pp. 661-666.
Alberts et al. Eds., “Molecular Biology of the Cell” 3rth Ed. Garland Publishing Inc., New York & London, (1994) pp. 160-162.
Andrea G. Bodnar et al., “Extension of Life-Span By Introduction of Telomerase Into Normal Human Cells” www.sciencemag.org, vol. 279, Jan. 16, 1998, pp. 349-352.
D.S. Mimitrov et al., “Topical Review—HIV Coreceptors,” The Journal of Membrane Biologg, Jun. 1, 1998, pp. 75-90.
Edward A. Berger, “HIV Entry and Tropism” Mechanisms of Lymphocyte Activation and Immune Regulation VII, 1998, pp. 151-157.
Adler Benjamin Aaron
MUSC Foundation for Research Development
Winkler Ulrike
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