Chemistry: analytical and immunological testing – Optical result – With fluorescence or luminescence
Patent
1989-06-09
1992-12-08
Yarbrough, Amelia Burgess
Chemistry: analytical and immunological testing
Optical result
With fluorescence or luminescence
435 29, 435173, 435968, 436800, C01N 2176
Patent
active
051697882
ABSTRACT:
Disclosed is a method of detecting a localized biochemical event in a cell, wherein said event results in a change in the membrane potential of that cell. The method includes treating the cell with a composition containing a lipophilic, cationic dye having a delocalized positive charge and the ability to undergo multiple changes in fluorescence spectra upon aggregation. The cell is treated with the dye for a time sufficient to enable the dye to associate with the cell. Dye which has not associated with the cell is then removed. Fluorescence is observed when the cell is exposed to light having a wavelength suitable for exciting the dye. The spectrum obtained is indicative of the relative membrane potential of said cell, and a change in that membrane potential being indicative of the occurrence of a biochemical event.
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Chen Lan B.
Smiley Stephen T.
Steele, Jr. Glenn D.
Burgess Yarbrough Amelia
New England Deaconess Hospital Corporation
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