Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1989-09-07
1992-11-10
Moskowitz, Margaret
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 4, 435 25, 435 29, 435190, 435968, 435973, 436 63, 436140, 436811, 436815, 436904, C12Q 132, C12Q 102, G01N 3348, C12N 904
Patent
active
051622033
ABSTRACT:
The present invention provides fluorescence-based methods for sensitively detecting total ADH activity in human sera and selectively measuring the activity of different classes of ADH in human sera and other body fluids and tissues. The present invention also provides highly purified Class I, Class II, and Class III isozymes, and methods for their purifiation. The class of substrates consisting of various naphthaldehydes and quinoline aldehydes provide the requisite sensitivity and selectivity for measurements of the activity of ADH and individual ADH classes. These fluorescence-based methods may serve as a diagnostic aid in disease assessment, in particular, diagnosis of alcohol abuse, alcoholism, alcohol consumption, altered alcohol sensitivity or tolerance.
REFERENCES:
Bernhard et al., Biochemistry 907:185-192 (1970).
Deetz et al., Biochemistry 23:6882-6828 (1984).
M.ang.rdh et al., PNAS (USA) 82:4979-4982 (1985).
Ibid., 83:8908-8912 (Dec. 1986).
Vallee et al., 1983, Isozymes: Current Topics in Biological and Medical Research, Liss, pp. 219-244, New York.
Montavon et al., Anal. Biochem. 176:48-56 (1989).
Wagner et al., Biochemistry 22:1857-1863 (1983).
Wagner et al., Biochemistry 23:2193-2199 (1984).
Ditlow et al., Biochemistry 23:6363-6368 (1984).
Strydom et al., Anal. Biochem. 123:422-429 (1982).
M.ang.rdh et al., Biochemistry, 25:7279-7282 (1986).
Kaiser et al., Biochemistry 27:1132-1140 (1988).
M.ang.rdh et al., Proc. Natl. Acad. Sci. USA 23:2836-2840 (1986).
Consalvi et al., Biochem. Biophys. Res. Commun. 139:1009-1016 (1986).
Vallee, Therap. Notes 73:71-74 (1966).
Bohman, Arch. Gen. Psychiat. 35:269-276 (1978).
Skursky et al., Drug and Alcohol Dependence 6:187-190 (1980).
Skursky et al., Anal. Biochem. 99:65-71 (1979).
Agarwal et al., Fresenius Anal. Chem. 311:374 (1982).
Kato et al., Clin. Chem. 30:1817-1820 (1984).
Khayrollah et al., Anal. Clin. Biochem. 19:35-42 (1982).
Keung et al., Anal. Biochem. 151:92-96 (1985).
Bosron et al., Biochemistry 18:1101-1105 (1979).
Dafeldecker and Vallee, J. Prot. Chem. 1:59 (1982).
Wierzchowski et al., 1989 Anal. Biochem. 178:57-62 (1989).
Moskowitz Margaret
President and Fellows of Harvard College
Zitomer Stephanie W.
LandOfFree
Methods of measuring isozymes and isozyme classes of alcohol deh does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods of measuring isozymes and isozyme classes of alcohol deh, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods of measuring isozymes and isozyme classes of alcohol deh will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2291746