Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1993-01-11
1995-06-20
Parr, Margaret
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 243, 536 245, 536 2431, 536 2432, C07H 2102, C07H 2104
Patent
active
054261805
ABSTRACT:
The present invention provides single-stranded circular oligonucleotides each with at least one parallel binding (P) domain and at least one corresponding anti-parallel binding (AP) domain separated from each other by loop domains. When more than one P or AP domain is included in a circular oligonucleotide of the present invention, the additional P or AP domains can constitute loop domains for a pair of corresponding P and AP domains, and vice versa. Each P and AP domain has sufficient complementarity to bind to one strand of a defined nucleic acid target wherein the P domain binds in a parallel manner to the target and the corresponding AP domain binds in an anti-parallel manner to the target. Moreover, the present single-stranded circular oligonucleotides can bind to both single-stranded and double-stranded target nucleic acids. The present invention also provides methods of making and using these oligonucleotides as well as kits and pharmaceutical compositions containing these oligonucleotides.
REFERENCES:
patent: 4766062 (1988-08-01), Diamond et al.
patent: 5176996 (1993-01-01), Hogan et al.
Lehninger, "Principles of Biochemistry", Worth Publishers, N.Y., 1982, p. 800.
Agrawal, et al. (1988), "Oligodeoxynucleoside Phosphoramidates and Phosphorothioates as Inhibitors of Human Immunodeficiency Virus," Proc. Natl. Acad. Sci. USA 85: 7079-7083.
Baumann, et al. (1988), "Interaction of DNA Hairpin Loops and a Complementary Strand by a Triplet of Base Pairs," Biochem. Biophys. Res. Commun. 157: 986-991.
Cooney, et al. (1988), "Site-Specific Oligonucleotide Binding Represses Transcription of the Human c-myc Gene in Vitro," Science 241: 456-459.
Durand, et al. (1992), "Triple-Helix Formation by an Oligonucleotide Containing One (dA).sub.12 and Two (dT).sub.12 Sequences Bridged by Two Hexaethylene Glycol Chains," Biochemistry 31: 9197-9204.
Erie, et al. (1989), "Melting Behavior of a Covalently Closed, Single-Stranded, Circular DNA," Biochemistry 28: 268-273.
Giovannangeli, et al. (1991), "Single-Stranded DNA as a Target for Triple-Helix Formation," J. Am. Chem. Soc. 113: 7775-7777.
Goodchild, et al. (1988), "Inhibition of Human Immunodeficiency Virus Replication by Antisense Oligodeoxynucleotides," Proc. Natl. Acad. Sci. USA 85: 5507-5511.
Griffin, et al. (1989), "Recognition of Thymine-Adenine Base Pairs by Guanine in a Pyrimidine Triple Helix Motif," Science 245: 967-971.
Lee, et al. (1984), "Poly(pyrimidine)-Poly(purine) Synthetic DNA's Containing 5-methylcytosine Form Stable Triplexes at Neutral pH," Nucleic Acids Research 12: 6603-6614.
Luebke, et al. (1989), "Nonenzymatic Ligation of Oligodeoxyribonucleotides on a Duplex DNA Template by Triple-Helix Formation," J. Am. Chem. Soc. 111: 8733-8735.
Mendel, et al. (1987), "Hoogsteen Base Pairs Proximal and Distal to Echinomycin Binding Sites on DNA," Proc. Natl. Acad. Sci. USA 84: 910-914.
Povsic, et al. (1989), "Triple Helix Formation by Oligonucleotides on DNA Extended to the Physiological pH Range," J. Am. Chem. Soc. 111: 3059-3061.
Riordan, et al. (1991), "Oligonucleotide-Based Therapeutics," Nature 350: 442-443.
Uhlmann, et al. (1990), "Antisense Oligonucleotides: A New Therapeutic Principle," Chemical Reviews 90: 543-584.
Vroom, et al. (1988), "Synthesis of Cyclic Oligonucleotides by a Modified Phosphotriester Approach," Nucleic Acids Research 18: 4607-4620.
Xodo, et al. (1990), "Spectroscopic and Calorimetric Investigation on the DNA Triplex Formed by d(CTCTTCTTTCTTTTCTTTCTTCTC) and d(GAGAAGAAAGA) at Acidic pH," Nucleic Acids Research 18: 3557-3564.
Horlick Kenneth R.
Parr Margaret
Research Corporation Technologies Inc.
LandOfFree
Methods of making single-stranded circular oligonucleotides does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods of making single-stranded circular oligonucleotides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods of making single-stranded circular oligonucleotides will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1845020