Methods of making pro-urokinase mutants

Details Methods of making pro-urokinase mutants Methods of making pro-urokinase mutants

2006-07-04

2006-07-04

Kerr, Kathleen M. (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S006120, C435S320100, C435S252100, C530S350000, C530S300000, C424S094630, C424S094640, C604S509000, C604S194000

Reexamination Certificate

active

07070958

ABSTRACT:
Methods are provided for producing non-glycosylated, single-chain and two-chain pro-urokinase (pro-UK) mutants. The methods include cultivating a specificE. colitype B strain that has been transformed with specific plasmids carrying a cDNA sequence that encodes pro-UK mutants and carries specific promoter sequences. Products produced by the methods have medical use for thrombolysis performed while sparing hemostatic clots, e.g., for particular applications such as after a stroke or heart attack.

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