Methods of making polymeric arrays

Details Methods of making polymeric arrays Methods of making polymeric arrays

1999-10-20

2001-01-09

Brusca, John S. (Department: 1631)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C435S006120, C536S023100, C536S024300

Reexamination Certificate

active

06171797

ABSTRACT:

INTRODUCTION
1. Technical Field
The field of this invention is nucleic acid arrays.
2. Background of the Invention
“Biochips” or arrays of binding agents, such as oligonucleotides and peptides, have become an increasingly important tool in the biotechnology industry and related fields. These binding agent arrays, in which a plurality of binding agents are deposited onto a solid support surface in the form of an array or pattern, find use in a variety of applications, including gene expression analysis, drug screening, nucleic acid sequencing, mutation analysis, and the like.
A critical feature of many arrays that have been developed is that each of the polymeric compounds of the array is stably attached to a discrete location on the array surface, such that its position remains constant and known through the use of the array. Stable attachment is achieved in a number of different ways, including covalent bonding of the polymer to the support surface and non-covalently interaction of the polymer with the surface. In many embodiments, it is desirable to covalently attach the binding agent to the surface of the solid support. A number of different protocols have been developed to provide for covalent attachment of binding agents, such as oligonucleotides, to a support surface, e.g. cross-linking of polymers to the surface, growing polymers from a support surface via stepwise reactions, and the like.
However, despite the number of different protocols that have been developed to date, there is continued interest in the development of new covalent attachment protocols. Of particular interest is the development of new protocols for producing nucleic acid arrays.
Relevant Literature
Patents and patent applications describing arrays of biopolymeric compounds and methods for their fabrication include: U.S. Pat. Nos. 5,242,974; 5,384,261; 5,405,783; 5,412,087; 5,424,186; 5,429,807; 5,436,327; 5,445,934; 5,472,672; 5,527,681; 5,529,756; 5,545,531; 5,554,501; 5,556,752; 5,561,071; 5,599,695; 5,624,711; 5,639,603; 5,658,734; WO 93/17126; WO 95/11995; WO 95/35505; EP 742 287; and EP 799 897. Also of interest are WO 97/14706 and WO 98/30575.
SUMMARY OF THE INVENTION
Methods and devices for producing arrays of binding agents covalently attached to the surface of a solid support are provided. In the subject methods, at least two distinct polymers of differing monomeric unit sequence are covalently attached to different locations on the surface of a solid support through a cycloaddition reaction, e.g. through the reaction of a diene with a dienophile. As such, the resultant arrays are characterized in that the polymers are attached to the solid support via a linking group that includes a cycloadduct. The subject methods are particularly suited for use in the preparation of nucleic acid arrays.


REFERENCES:
patent: 5242974 (1993-09-01), Holmes
patent: 5384261 (1995-01-01), Winkler et al.
patent: 5405783 (1995-04-01), Pirrung et al.
patent: 5412087 (1995-05-01), McGall et al.
patent: 5424186 (1995-06-01), Fodor et al.
patent: 5429807 (1995-07-01), Matson et al.
patent: 5436327 (1995-07-01), Southern et al.
patent: 5445934 (1995-08-01), Fodor et al.
patent: 5472672 (1995-12-01), Brennan
patent: 5527681 (1996-06-01), Holmes
patent: 5529756 (1996-06-01), Brennan
patent: 5545531 (1996-08-01), Rava et al.
patent: 5554501 (1996-09-01), Coassin et al.
patent: 5556752 (1996-09-01), Lockhart et al.
patent: 5561071 (1996-10-01), Hollenberg et al.
patent: 5580697 (1996-12-01), Keana et al.
patent: 5599695 (1997-02-01), Pease et al.
patent: 5624711 (1997-04-01), Sundberg et al.
patent: 5639603 (1997-06-01), Dower et al.
patent: 5658734 (1997-08-01), Brock et al.
patent: 0 742 287 A2 (1996-11-01), None
patent: 0 799 897 A1 (1997-10-01), None
patent: 93/17126 (1993-09-01), None
patent: 95/11995 (1995-05-01), None
patent: WO 95/25116 (1995-09-01), None
patent: 95/35505 (1995-12-01), None
patent: 97/14706 (1997-04-01), None
patent: 98/30575 (1998-07-01), None
“Large Scale Oligonucleotide Synthesis,” Oct. 28-29, 1997 at Westin Horton Plaza Hotel, San Diego, California.

Affiliated with

Also associated with

Rating

Methods of making polymeric arrays does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods of making polymeric arrays, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods of making polymeric arrays will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2533743