Methods of making polyhydroxyalkanoates comprising 4-hydroxybuty

Details Methods of making polyhydroxyalkanoates comprising 4-hydroxybuty Methods of making polyhydroxyalkanoates comprising 4-hydroxybuty

1998-02-12

2000-09-12

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

435141, 435146, 435170, C12P 762, C12P 752, C12P 742, C12P 104

Patent

active

061176583

ABSTRACT:
The PHA biosynthetic pathway is combined with a succinic semialdehyde metabolic pathway that metabolizes succinic semialdehyde via a 4-hydroxybutyryl-CoA intermediate in order to produce high levels of PHA comprising 4HB monomer units. This combination is particularly useful, in part, because the PHA biosynthetic pathway is well known and has been expressed to produce P(3HB) to levels as high as 70-80% of the cell dry weight, while the succinic semialdehyde metabolic pathway is also well known, and the combination of the two pathways means that production of PHAs comprising 4HB monomer units does not depend on immediate precursors of 4-hydroxybutyrate. Rather, the production of the PHAs comprising 4HB monomer units can occur simply from the extraction of succinate or succinyl-CoA from the citric acid cycle when the host cell is grown on an inexpensive carbon source such as glucose.

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