Methods of making L-amino acids in coryneform using the sigE...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...

Reexamination Certificate

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C435S115000

Reexamination Certificate

active

06913908

ABSTRACT:
The present invention relates lo an isolated polynucleotide fromCorynebacterium glutamicumcomprising a polynucleotide sequence chosen from the group c insisting of (a) a polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO: 2; (b) a polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO: 2; (c) a polynucleotide which is complementary to the polynucleotides of(a) or (b), and (d) a polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of (a), (b), or (c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the sigE gene is present in enhanced form, and the use of polynucleotides which comprise the sequence according to the invention as hybridization probes.

REFERENCES:
patent: 2002/0197605 (2002-12-01), Nakagawa et al.
patent: 195 48 222 (1997-06-01), None
patent: 0864 654 (1998-09-01), None
patent: 1 108 790 (2001-06-01), None
Database EMBL; Accession No. MSU87307, Mycobacterium smegnatis sigE gene (May, 1997).
Wu et al., “A mycobacterial extracytoplasmic function sigma factor involved in survival following stress”, Journal of Bacteriology, vol. 179, No. 9, 1997, p. 2922-2929.
Eggeling et al., “L-glutamate and L-lysine: traditional products with impetuous developments”, Appl. Microbiolog. Biotechnol. vol. 52, 1999, p. 146-153.

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