Methods of making fluorescent microspheres

Details Methods of making fluorescent microspheres Methods of making fluorescent microspheres

1989-11-08

1993-03-16

Stoll, Robert L.

Coating processes

Particles, flakes, or granules coated or encapsulated

Solid encapsulation process utilizing an emulsion or...

525 541, 25230135, 436533, 436532, 436546, 436800, 428407, 530402, 530816, G01N 33547, C08F 810

Patent

active

051943000

ABSTRACT:
Highly fluorescent latex microspheres have a diameter of less than five hundred angstroms and have more than five thousand fluorescent markers per sphere. The microspheres are prepared by reacting an acrylic latex bead with a diamine and a fluorescent amine at elevated pH. A protein such as avidin or an immunoglobulin may then be conjugated to the diamine. A single fluorescent microsphere is visible using standard fluorescent microscopy. Therefore the microspheres may be utilized not only to visualize cell surface anitgens but also DNA encoding for single genes, by means of a biotinylated DNA probe.

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