Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-05-01
1998-06-30
LeGuyader, John L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 911, 435 9121, 435 913, 435 9131, 435 914, 435 9142, 4351721, 4351723, 4352523, 4352542, 43525421, 4353201, 536 231, C12P 2100, C12P 1934, C12N 1500, C07H 2102
Patent
active
057732442
ABSTRACT:
A prototype RNA cyclase ribozyme that allows efficient production of circular RNA. Methods for modifying the prototype to produce a wide variety of custom circular RNA are detailed. The method utilizes a new plasmid which enables production of a wide variety of imaginable RNA sequences in a covalent, circular form free from intron sequences in vitro. At a particular site in the plasmid, a sequence coding for the desired circular RNA is inserted to create a new RNA cyclase ribozyme gene. RNA transcribed from RNA cyclase ribozyme genes autocatalytically converts the desired RNA sequence it contains into circular form. RNA cyclase genes may be placed into appropriate expression vectors for synthesis of circular RNA in vivo as part of ribozyme or antisense gene regulation approaches to genetic engineering.
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Ares, Jr. Manuel
Ford Ethan E.
Larson Thomas G.
LeGuyader John L.
Regents of the University of California
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