Multicellular living organisms and unmodified parts thereof and – Method of making a transgenic nonhuman animal – Involving breeding to produce a double transgenic nonhuman...
Reexamination Certificate
2000-01-28
2004-05-25
Paras, Peter (Department: 1632)
Multicellular living organisms and unmodified parts thereof and
Method of making a transgenic nonhuman animal
Involving breeding to produce a double transgenic nonhuman...
C800S008000, C424S198100, C530S399000
Reexamination Certificate
active
06740794
ABSTRACT:
FIELD OF THE INVENTION
A technique for in vitro secretion of androgenic sex hormone (AH) from the androgenic gland (AG) of fresh water male prawn in a defined culture medium is disclosed. AH finds use in the manipulation of the reproductive processes of prawns and shrimp for the production of sex-skewed or mono-sex progeny.
BACKGROUND OF THE INVENTION
Sexual differentiation and gametogenesis in the decapods, marine shrimp (genus Penaeus) and freshwater prawns (genus Macrobrachium), occurs, respectively, in the anatomically separate testes and Androgenic Hormone (AH)-producing Androgenic Gland (AG).
Nagamine et al. (1980b) Gen. Comp. Endocrin. 41:423-441 partially sex-reversed genetic male decapods (prawns) to phenotypic females by AG ablation. This was subsequently confirmed by Sagi and Cohen (1991). Nagamine et al. (1980a) Gen. Comp. Endocrin. 41:442-457 partially sex-reversed genetic female prawns to “neomales” by AG implantation. Malecha et al. (1992a) Aquaculture 105:1-18 extended this work to smaller female recipients and achieved sex-reversal by AG implantation such that neomales produced skewed sex ratio, predominantly female, progeny when mated with normal females.
The surgical implantation of AG tissue in genetic females to produce neomales is time consuming, tedious, and is not amenable to large-scale practices. Accordingly, it is an object of the present invention to provide isolated shrimp or prawn AH. It is also an object to provide methods for use of AH in the production of neomales from genetic females and sex-skewed or mono-sex shrimp or prawn progeny, therefrom.
SUMMARY OF THE INVENTION
In accordance with the foregoing object, the present invention provides an isolated shrimp or prawn androgenic polypeptide and methods of use.
Accordingly, the invention provides neomale shrimp and prawns which do not contain transplanted androgenic gland tissue.
In another aspect, the invention provides methods of producing neomale shrimp and prawns.
In yet another aspect, the invention provides methods of producing a population of shrimp and prawns having a skewed percentage in favor of females to males.
In a further aspect, the invention provides a method of identifying an agent that binds or modulates the biological activity of an androgenic polypeptide of shrimp or prawns. Accordingly, the invention provides agents that bind to or modulate an androgenic polypeptide of a shrimp or protein.
Other embodiments or aspects of the invention will become apparent to the skilled artisan by the following description of the invention.
REFERENCES:
patent: 5314882 (1994-05-01), Pantic et al.
patent: 0 514 015 (1992-11-01), None
Nakashima; Reproductive Stratigies in a Partially Protandrous Shrimp,Athanas kominatoensis(Decapoda: Alpheidae): Sex Change as the Best of a Bad Situation for Subordinates, 1987, J. Ethol. 5: 145-159.*
Martin et al. “Purification and characterization of Androgenic Hormone from the Terrestrial IsopodArmadillidium vulgare Latr. (Crustacea, Oniscidea),” Gen Comp. Endocrinol. 80(3):349-354 (1990).
Mohamed et al. “Effect of androgenic gland ablation on sexual characters of the male Indian whit prawnPenaeus indicusH. Milne Edwards,” Indian J. Exp. Biol. 29(5): 478-480 (1991).
Nagamine et al. “Effects of androgenic gland ablation on the male sexual characteristics in the freshwater prawn,” Am. Zool 17(4):968 (1977).
Nagamine et al. “Effects of androgenic gland ablation on male primary and secondary sexual characteristics in the Malaysian Prawn,Macrobrachium rosenbergiii(de Man) (Decapods, Palaemonidae), with First Evidence of Induced Feminization in a Nonhermaphroditic Decapod,” Gen. Comp. Endocrinol 41(4) 423-441 (1980).
Nagamine. Annual Meeting of the American Society of Zoologists, Society of Systematic Zoology and the American Microscopical Society, Tampa, Fla., Dec. 27-30, “Cloning and expression of seminal vesicle specific peptide of terrestrial isopod,Armadillidium vulgare,” Endocrinology, 8: abstract.
Negishi et al. “Alterations in the integument ofArmadillidium vulgaremasculinized by implantation of androgenic glands,” Invertebrate Reproduction and Development, 21(3):179-186 (1992).
Negishi et al. Sixty-Second Annual Meeting of the Zoological Society of Japan, Oct. 13-15 (1991). Endocrinology, 1183: abstract.
Okuno et al. “Characterization and cDNA cloning of androgenic gland hormone of the terrestrial isopodArmadillidium vulgare,” Biochem. Biophys. Res. Commun. 264(2):419-423 (1999).
Okuno et al. “Purification and properties of androgenic gland hormone from the terrestrial isopodArmadillidium vulgare,” Zoological Science (Tokyo) 14(5):837-842 (1997).
Okuno et al. “Purification and properties of the androgenic gland hormone from the terrestrial isopod,” Endocrinology, 7, abstract.
Payen et al. 11th conference European Comparative Endocrinologists, Jerusalem, Israel, Aug. 10-14 (1981). Gen Comp Endocrinol 46(3) (1982).
Payen. C R Hebd Seances Acad Sci Ser D Sci Nat. 280(9):1111-1114 (1975).
Raimond et al. General and Comparative Endocrinology, 50(1): 146-155 (1983), English abstract.
Sagi et al. Revista da Sociedade Brasileira de Zootecnia, 24(2):300-309 (1995), English abstract.
Sagi et al. “Intersex Red Claw Crayfish,Cherax quadricarinatus(von Martens): Functional males with pre-vitellogenic ovaries,”Biological Bulletin. 190(1): 16-23 (1996).
Sagi et al. “Effect of androgenic gland ablation on morphotypic differentiation and sexual characteristics of male freshwater prawns,Macrobrachium rosenbergii,” Gen Comp Endocrinol 77(1):15-22 (1990).
Nagamine et al., “Masculinization of FemaleMacrobrachium rosenbergil(de Man) (Decapoda, Palaemonidae) by Androgenic Gland Implantation,” General and Comparative Endocrinology, 41:442-457 (1980).
Malecha et al., “Sex Ratios and Sex-determination in progeny from crosses of surgically sex-reversed freshwater prawns,Macrobrachium rosenbergii,” Aquaculture, 105:201-218 (1992).
Bonilla et al., Rev. Biol. Trop 42(suppl. 2):121-129 (1994), English abstract.
Chang et al., “Prostatic Spemine-binding Protein,” J. Biol. Chem. 262(6):2826-2831 (1987).
Fowler et al. “The structure and function of the androgenic gland in Cherax destructor (Decapoda: Parastacidae,” Aquaculture, 171(1-2):135-148 (1999).
Hasegawa et al. “Masculinization of Female by the newly-formed Androgenic Glands in the ZW and WW females of the Isopod Crustacean,” Zool Sci (Tokyo), 2(3):419-422 (1985).
Hasegawa et al. “Isolation and Properties of Androgenic Gland Hormone from the Terrestrial Isopod,Armadillidium vulgare,” Gen. Comp. Endocrinol. 67(1):101-110 (1987).
Hasegawa et al. “Masculinization of WW females in the Isopod Crustacean,Armadillidium vulgare,” Annot Zool Jpn. 56(3):163-166 (1984).
Juchault et al. General and Comparative Endocrinology, 36(2):175-186 (1978), English abstract.
Katakura et al. “Purification of androgenic gland hormone from the terrestrial isopod,Armadillidium vulgare(Latreille, 1804),” Monitore Zoologico Italiano (Italian Journal of Zoology No. 4) 351-358 (1989).
Katakura et al. “Masculinization of females of the Isopod Curstacean,Armadillidium vulgare, following injections of an active extract of the Androgenic Gland,” Gen Comp. Endocrinol. 48: 57-62 (1983).
Katakura et al. “Isolation of androgenic gland hormone by high performance liquid chromatography,” Endocrinology, P. 962, EN11. 55th Annual Meeting of the Zoological Society of Japan, Morioka. Sep. 27-29 1984. Zool Sci. (Tokyo) 1(6) 1984.
Legrand-Hamelin. C R Seances Soc Biol Fil 171(1):176-180 (1977), English abstract.
Martin et al. “The structure of a glycosylated protein hormone responsible for sex determination in the isopod,Armadillidium vulgare,” Eur. J. Biochem. 262(3):727-736 (1999).
Sagi et al. American Zoologist, 36(5):10a (1996).
Sagi et al. “Sexual differentiation in decapod crustaceans: role of the androgenic gland,” Invertebrate Reproduction and Development. 31(1-3):55-61 (1997).
Sagi et al. American Zoologist 37(5):183a (1997).
Suzuki, S. “Sex-Reversal of maleArmadillidium vulgare(Isopods, Malacostraca, Crustacea) following andrectomy and partial Gonadectomy,” Gen Comp
Malecha Spencer R.
Sun Piera S.
Dorsey & Whitney
Paras Peter
Ropp Traci H.
Trecartin Richard F.
University of Hawai'i
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