Methods of introducing foreign DNA into cells

Details Methods of introducing foreign DNA into cells Methods of introducing foreign DNA into cells

1994-12-23

1997-01-21

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 691, 435 701, 435 703, 4352402, 4352523, 536 231, 935 34, 935 52, 935 70, C12N 1590, C12N 1579, C12N 1587, C07H 2104

Patent

active

055958990

ABSTRACT:
The present invention provides an easy and efficient method of introducing a foreign DNA into certain cells which have been considered difficult to be transformed by conventional methods.
The present inventors have found that bis-benzimdazolyl compounds which specifically bind to DNA were useful as a carrier to introduce the DNA into a target cell. Foreign DNA was introduced into a host cell by exposing said cell to a suspension or a solution containing the foreign DNA-compound complex.

REFERENCES:
Cotten et al. "Transferrin-Polycation Mediated Introduction of DNA Into Human Leukemic Cells . . . " Proc. Natl. Acad Sci. 87: 4033-4037 1990.
McNeil et al. "Glass Beads Load Macromolecules Into Living Cells" J. Cell Science 88:669-678 1987.
Zimmer et al. "Non-Intercalating DNA-Binding Ligands: Specificity of The Interaction & Their Use as Tools . . . " Biophys Molec. Biol. 47(1) 31-112 1986.
A Mammalian Cell Mutant With Enhanced Capacity to Dissociate a Bis-Benzimidazole Dye-DNA Complex, P. J. Smith et al., Carcinogenesis, 9(3):485-490 (1988).
Wagner et al., "Transferrin-polycation conjugates as carriers for DNA uptake into cells", Proc. Natl. Acad. Sci. USA 87:3410-3414 (1990).
Brackett et al., "Uptake of Heterologous Genome by Mammalian Spermatozoa and Its Transfer to Ova through Fertilization", Proc. Natl. Acad. Sci. 68:353-357 (1971).
Hahn et al., "Elimination of Plasmidic Determinants by DNA-complexing Compounds", Top. Infect. Dis.-Drug Recept. Interact. Antimicrob. Chemother. Symp. 1:99-113 (1974).
Lavitrano et al., "Sperm Cells as Vectors for Introducing Foreign DNA into Eggs: Genetic Transformation of Mice", Cell 57:717-723 (1989).

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