Methods of inducing immune tolerance using immunotoxins

Details Methods of inducing immune tolerance using immunotoxins Methods of inducing immune tolerance using immunotoxins

1997-04-15

2000-08-15

Hutzell, Paula K.

Drug, bio-affecting and body treating compositions

Conjugate or complex of monoclonal or polyclonal antibody,...

Conjugated to proteinaceous toxin or fragment thereof

4241841, A61K 39395, A61K 3900

Patent

active

061032352

ABSTRACT:
Provided is a method of treating an immune system disorder not involving T cell proliferation, comprising administering to the animal an immunotoxin comprising a mutant diphtheria toxin moiety linked to an antibody moiety which routes by the anti-CD3 pathway, or derivatives thereof under conditions such that the disorder is treated. Thus, the present method can treat graft-versus-host disease. Also provided is a method of inhibiting a rejection response by inducing immune tolerance in a recipient to a foreign mammalian donor tissue or cells, comprising the steps of: a) exposing the recipient to an immunotoxin so as to reduce the recipients's peripheral blood T-cell lymphocyte population by at least 80%, wherein the immunotoxin is anti-CD3 antibody linked to a diphtheria protein toxin, wherein the protein has a binding site mutation; and b) transplanting the donor cells into the recipient, whereby a rejection response by the recipient to the donor organ cell is inhibited, and the host is tolerized to the donor cell.

REFERENCES:
patent: 5167956 (1992-12-01), Neville, Jr.
Ma, et al. Expression and Characterization of a Divalent Chimeric Anti-Human CD3 Single Chain Antibody Scand. J. Immunol. 43, 134-139, 1996.
Lu et al (J. Am. Soc. Neprol., 4:1239-1256, 1993.
Nemoto et al (Agents Actions, 36:306-311, 1992.
Koehler et al (Bone Marrow Transplantation, 13:571-575, 1994.
Neville et al. J. Immunotherapy 19(2):85-92, 1996.
Thompson et al. J. Biol. Chem. 270(47):28037-28041, Nov. 24, 1995.
Neville et al. J. Controlled Release 24(1-3):133-144, May 1993.
Neville et al. Proc. Natl. Acad. Sci. USA 89:2585-2589, 1992.
Pastan et al. Science 254:1173-1177, Nov. 22, 1991.
Parlevliet et al. Transplantation 50:889-892, Nov., 1990.
Kappler et al. Science 244:811-813, May 19, 1989.
Greenfield et al. Science 238:536-539, 1987.
Nooij and Jonker Eur. J. Immunol. 17:1089-1093, 1987.
Thomas et al. Transplantation (1994) 57:101-115.
Thomas et al. Transplantation Proceedings (1995) 27:3167.
Traunecker, et al. Bispecific single chain molecules (Janusins) target cytotoxic lymphocytes on HIV infected cells. The EMBO Journal (1)12:3655-3659 (1991).
Hayden et al. Single-chain mono-and bispecific antibody derivatives with novel biological properties and antitumor activity from a COS cell transient expression system. Therapeutic Immunology(1):3-15 (1994).

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