Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2007-08-21
2007-08-21
Allen, Marianne P. (Department: 1647)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S069520, C530S350000, C530S351000, C930S144000
Reexamination Certificate
active
10496218
ABSTRACT:
The present invention relates to methods of increasing protein expression levels whereby at least one amino acid in a protein amino acid sequence is substituted for the amino acid, proline. Preferably, the substitution occurs within 15 amino acids, more preferably within 10 amino acids and most preferably within 5 amino acids of a cysteine amino acid residue. The present invention not only includes methods for polypeptides with proline substitutions, but also polynucleotides with codon substitutions for which a codon for any amino acid, except proline, is substituted for a codon encoding for proline.
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Brondyk William
Jiang Xuliang
Schweickhardt Rene Lynn
Allen Marianne P.
Applied Research Systems Ars Holding N.V.
Howrey LLP
Woodward Cherie
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