Methods of increasing oil content of seeds

Details Methods of increasing oil content of seeds Methods of increasing oil content of seeds

1996-07-01

1999-07-20

McElwain, Elizabeth F.

Multicellular living organisms and unmodified parts thereof and

Plant, seedling, plant seed, or plant part, per se

800264, 800281, 800306, 800312, 435 691, 435468, 435419, 536 236, A01H 500, C12N 1582

Patent

active

059258055

ABSTRACT:
An Arabidopsis acetyl-CoA carboxylase (ACCase) gene has been isolated and sequenced. The 10-kb gene encodes a 251-kd cytosolic ACCase isozyme. The nucleic acid sequence of the gene is set forth in SEQ ID No. 1 and has been accorded GenBank Accession No. L27074. The cDNA sequence and deduced amino acid sequence of the cDNA are set forth in SEQ ID Nos. 2 and 3, respectively. By controlling expression of the gene of the present invention, carboxylation of acetyl-CoA to produce malonyl-CoA may be controlled. Thus, by introducing constructs of the gene of the present invention in sense or anti-sense orientation, carboxylation of acetyl-CoA to produce malonyl-CoA may be increased or decreased. Consequently, fatty acid synthesis and elongation in plants and seeds which depends on malonyl-CoA may also be controlled.

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