Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-05-15
2007-05-15
Nashed, Nashaat T. (Department: 1656)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S471000, C435S486000, C435S252350, C536S023700, C536S023100
Reexamination Certificate
active
10017471
ABSTRACT:
Methods are provided for increasing and altering the timing of antibiotic production inStreptomycesspecies, particularlyS. coelicolorandS. lividans, by functionally deleting theS. coelicolorscbA and scbR genes, respectively, or their homologues. Also provided are strains having such mutations, and methods of producing antibiotics using such strains. Also provided are methods for identifying strains in which functional deletion of the scbA and/or scbR genes or their homologues leads to the above effects.
REFERENCES:
Kawachi et al. Identification of an AfsA homologue (BarX) fromStreptomyces virginiaeas a pleiotropic regulator controlling autoregulator biosynthesis, virginiamycin biosynthesis and virginiamycin M1 resistance. Mol Microbiol (Apr., 2000) 36:302-313.
Takano et al. A complex role for the gamma-butyrolactone SCB1 in regulating antibiotic production inStreptomyces coelicolorA3(2). Molecular Microbiology (2001) 41(5):1015-1028.
Butler et al. Deletion of scbA enhances antibiotic production inStreptomyces lividans. Appl. Microbiol. Biotechnol. (2003) 61:512-516.
GenBank Accession No. NP—823445. putative gamma-butyrolactone biosynthesis protein [Streptomyces avermitilisMA-4680] (2004).
Ando, N., et al., “Involvement of AfsA in A-factor Biosynthesis as a Key Enzyme”, J. Antibiot. 50: 847-852, (1997).
Bate, N., et al., “Multiple regulatory genes in the tylosin biosynthetic cluster ofStreptomyces fradiae”, Chemistry & Biology, 6: 617-624, (1999).
Fouces, R., et al., “The tylosin biosynthetic cluster fromStreptomyces fradiae: genetic organization of the left region”, Microbiology, 145: 855-868, (1999).
Gramajo, H.C., et al., “Stationary-phase production of the antibiotic actinorhodin inStreptomyces coelicolorA3(2) is transcriptionally regulated”, Mol. Microbiol., 7: 837-845, (1993).
Hara, O., et al., “Genetic Analysis of A-factor Synthesis inStreptomyces coelicolorA3(2) andStreptomyces griseus”, J. Gen. Microbiol., 129: 2939-2944, (1983).
Hopwood, D.A., et al., “Genetics of Antibiotic Production inStreptomyces coelicolorA3(2), a ModelStreptomycete”, In: Genetics and Biochemistry of Antibiotic Production, Vining, L. (ed), Butterworth-Heinemann, Newton, MA, USA, pp. 65-102 (1985).
Hourinouchi, S., and Beppu, T., Autoregulators. In: Genetics and Biochemistry of Antibiotic Production. Vining, L. (ed) Butterworth-Heinemann, Newton, MA, USA. pp. 103-119, (1994).
Horinouchi, S., et al., “The ClonedSteptomycesBikiniensis A-Factor Determinant”, J. Antibiot., 36: 636-641, (1985).
Horinouchi, S., et al., “Nucleotide Sequence and Transcriptional Analysis of theStreptomyces griseusGene (afsA) Responsible for A-Factor Biosynthesis”, J. Bacteriol., 171: 1206-1210, (1989).
Ikeda, H., et al., “Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin inStreptomyces avermitilis”, Proc. Natl. Acad. Sci. 17: 9509-9514, (1999).
Kieser, H.M., et al., “A Combined Genetic and Physical Map of theStreptomyces coelicolorA3(2) Chromosome”, J. Bacteriol. 174: 5496-5507, (1992).
Kim, H.S., et al., “Identification of binding protein of Virginiae butanolide C, an autoregulator in Virginiamycin production, fromStreptomyces Virginiae”, J. Antibiot., 42: 769-778, (1989).
Kinoshita, H., et al., “Butyrolactone Autoregulator Receptor Protein (BarA) as a Transcriptional Regulator inStreptomyces virginiae”, J. Bacteriol. 179: 6989-93, (1997).
Kitani, S., et al., “In vitro analysis of the butyrolactone autoregulator receptor protein (FarA) ofStreptomyces lavendulaeFRI-5 reveals that FarA as a DNA-binding transcriptional regulator that controls its own synthesis”, J. Bacteriol., 181: 5081-5084, (1999).
Lezhava, A., et al., “Chromosomal deletions inStreptomyces griseusthat remove the afsA locus”, Mol. Gen. Genet. 253: 478-483, (1997).
Miyake, K., et al., “The A-factor-binding protein ofStreptomyces griseusnegatively controlsStreptomycinproduction and sporulation”, J. Bacteriol., 172: 3003-3008, (1990).
Mori, K., “Revision of the absolute configuration of A-factor”, Tetrahedron Lett. 39: 3107-3109, (1983).
Nakano, H., et al., “Gene replacement analysis of theStreptomyces virginiaebarA gene encoding the butyrolactone autoregulator receptor that barA acts as a repressor inVirginiamycin biosynthesis”, J. Bacteriol., 180: 3317-3322, (1998).
Nihira, T., et al., “Structure-activity relationships ofVirginiae butanolideC, an inducer ofVirginiamycinproduction inStreptomyces Virginiae”, J. Antibiot., 41: 1828-1837, (1988).
Okamoto, S., et al., “Virginiae butanolidebinding protein fromStreptomyces virginiae”, J. Biol. Chem., 270: 12319-12326, (1995).
Onaka, H., et al., “Cloning and characterization of the A-Factor receptor gene fromStreptomyces griseus”, J. Bacteriol., 177: 6083-6092, (1995).
Onaka, H., et al., “DNA-binding activity of the A-factor receptor protein and its recognition DNA sequences”, Mol. Microbiol., 24: 991-1000, (1997).
Onaka, H., et al., “Involvement of two A-factor receptor homologues inStreptomyces coelicolorA3(2) in the regulation of secondary metabolism and morphogenesis”, Mol. Microbiol. 28: 743-753, (1998).
Ohnishi, Y., et al., “The A-factor regulatory cascade leading tostreptomycin biosynthesisinStreptomyces griseus: identification of a target gene of the A-factor receptor”, Mol. Microbiol., 34: 102-111, (1999).
Redenbach, M., et al., “A set of ordered cosmids and a detailed genetic and physical map for the 8 MbStreptomyces coelicolorA3(2) chromosome”, Mol. Microbiol., 21: 77-95, (1996).
Ruengjitchatchawalya, M., et al., “Purification and characterization of the IM-2-binding protein fromStreptomyces sp.strain FRI-5”, J. Bacteriol., 177: 551-557, (1995).
Sato, K., et al., “Isolation and structure of a new butyrolactone autoregulator fromStreptomyces sp.FRI-5”, J. Ferment Bioeng., 68: 170-173, (1989).
Takano, E., et al., “Transcriptional regulation of the redD transcriptional activator gene accounts for growth-phase-dependent production of the antibiotic undeclyprodigiosin inStreptomyces coelicolorA3(2)”, Mol. Microbiol., 6: 2729-2804, (1992).
Takano, E., et al., “Purification and structural determination of SCB1, a γ-butyrolactone that elictis antibiotic production inStreptomyces coelicolorA3(2)”, J. Biol. Chem., 275: 11010-11016, (2000).
Waki, M., et al., “Cloning and characterization of the gene (farA) encoding that receptor for an extracellular regulatory factor (IM-2) fromStreptomyces sp.strain FRI-5”, J. Bacteriol., 179(16): 5131-5137, (1997).
Yamada, Y., “Autoregulatory factors and regulation of antibiotic production inStreptomyces”, In Microbial singalling and communication. England, R., Hobbs, G., Bainton, N., and Roberts, D. McL. (eds.) Cambridge: the Society for General Microbiology, pp. 177-196, (1999).
Yamada, Y., et al., “The structure of inducing factors forVirginiamycinproduction inStreptomyces virginiae”, J. Antibiot., 40: 496-504, (1987).
Passantio, R., et al., “Additional copies of the actII regulatory gene induce actinorhodin production in pleiotropic bld mutants ofStreptomyces coelicolorA3(2)”, J. Gen. Microbiol., 137: 2059-2064, (1991).
Aigle, B., et al., “A single amino acid substitution in region 1.2 of the principal α factor ofStreptomyces coelicolorA3(2) results in pleiotropic loss of antibiotic production”, Mol. Microbiol., 37 (5): 995-1004 (2000).
Sugiyama, M., et al., “Site-directed mutagenesis of the A-factor receptor protein: Val-41 important for DNA-binding and Trp-119 important for ligand-binding”, Gene, 222(1): 133-44, (1998).
Bibb Mervyn J.
Takano Eriko
Dann Dorfman Herrell & Skillman P.C.
Nashed Nashaat T.
Plant Bioscience Limited
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