Methods of increasing accumulation of essential amino acids in s

Details Methods of increasing accumulation of essential amino acids in s Methods of increasing accumulation of essential amino acids in s

1997-03-26

2000-10-03

Smith, Lynette F.

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

800298, 435419, 435468, 4353201, 435 691, 536 241, 536 236, C12N 1500, C12N 1529, C12N 1582, A01H 400

Patent

active

061276006

ABSTRACT:
The present invention provides a method for increasing the levels of essential amino acids in seeds of plants, thereby enhancing the nutritional value of the seeds. The method comprises manipulating the metabolic pathway of the amino acid to provide an increased source of the target free amino acid and, concomitantly, over-expressing a preselected gene coding for the protein containing the target amino acid, such that there is accumulation of protein-bound target amino acid. A complementary protein sink is thus produced. The present invention is particularly useful in increasing levels of methionine, lysine and threonine in seeds.

REFERENCES:
Smith et al. Nature. vol. 334, pp. 724-726, Aug. 1988.
Napoli et al. The Plant Cell. vol. 2, pp. 279-289, Apr. 1990.
Karchi et al. The Plant Journal. vol. 3, No. 5, pp. 721-727, 1993.
Altenbach et al. Plant Molecular Biology. vol. 13, pp. 513-522, 1989.
Altenbach et al., "Manipulation of methionine-rich protein genes in plant seeds", Trends in Biotechnology, vol. 8, No. 6, pp. 156-160 (1990).

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