Methods of in situ hybridization

Details Methods of in situ hybridization Methods of in situ hybridization

1997-06-13

1999-09-07

Myers, Carla J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435 405, 435 4052, 435455, 435325, 536 231, 536 243, 536 2431, C12Q 168, C07H 2104

Patent

active

059486171

ABSTRACT:
A method and single solution denaturation and hybridization solution useful with synthetic oligonucleotide probes for hybridizing DNA or RNA sequences by in situ hybridization. The inventive protocol employs synthetic oligonucleotide probes and a glycerol-based hybridization solution which is particularly useful in fluorescence labeling of chromosomes X, Y, 13, 18 and 21 in human lymphocytes, amniocytes and metaphase chromosomes, with the label being sufficiently stable to permit archival storage of the labeled sample for later hybridization or analysis.

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