Methods of identifying non-specific inhibitors of biomolecules

Details Methods of identifying non-specific inhibitors of biomolecules Methods of identifying non-specific inhibitors of biomolecules

2005-05-03

2005-05-03

Saucier, Sandra E. (Department: 1651)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Reexamination Certificate

active

06887658

ABSTRACT:
The present invention provides methods of identifying compounds that non-specifically inhibit biological reactions. The present invention further includes kits that facilitate this identification. In addition, complications of compounds for use in high throughput drug screening that have been evaluated by the disclosed methodology are also part of the present invention. Further, the invention provides methods for identifying a false positive compound previously identified as positive in a screening assay by measuring the activity of at least one biological activity in the presence and absence of a small molecule compound capable of inhibiting aggregate formation, e.g., digitonin.

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Luo et al., Molecular and Cellular Biology, 21:5678-5687 (2001).
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Roche et al., J. Med. Chem., 45:137-142 (2002).
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