Methods of identifying inhibitors of sensor histidine kinases th

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase

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435194, 436 86, 530300, 530350, C12Q 148, G01N 3300

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06077682&

ABSTRACT:
The present invention provides N-terminal truncated transmembrane sensor histidine kinases that retain their ability to be autophophorylated and/or their related histidine kinase activity. The N-terminal truncated transmembrane sensor histidine kinases are useful for obtaining detailed three-dimensional structural data of the catalytic portion of the protein. The three-dimensional structural data is included as part of the invention. In addition, the present invention provides methodology for related structure based rational drug design using the three-dimensional data. Nucleotide and amino acid sequences of the N-terminal truncated transmembrane sensor histidine kinases are also provided.

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