Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-12-12
1998-12-22
McKelvey, Terry A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C12Q 168
Patent
active
058517731
ABSTRACT:
This invention provides a method of isolating a progression suppressor gene which suppresses expression of a gene regulated by a long terminal repeat of a retrovirus comprising: a) introducing DNA containing a long terminal repeat regulated protein kinase C .beta..sub.1 gene into a population of transformed cells; b) treating the cells from step (a) so as to induce the expression of at least one progression suppressor gene and thereby cause a subpopulation of the cells to selectively suppress the expression of the long terminal repeat regulated protein kinase C .beta..sub.1 gene; c) selecting cells which express the progression suppressor gene; d) isolating mRNA from the cells from step (c); e) comparing the mRNA so obtained with mRNA obtained from the uninduced transformed cells so as to identify mRNA only expressed by the cells from step (c); and f) isolating the gene encoding the mRNA in step e) so as to thereby identify the progression suppressor gene.
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Fisher Paul B.
Su Zao-zhong
McKelvey Terry A.
The Trustees of Columbia University in the City of New York
White John P.
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