Combinatorial chemistry technology: method – library – apparatus – Method of screening a library – By measuring the effect on a living organism – tissue – or cell
Reexamination Certificate
2004-03-26
2010-11-09
Lundgren, Jeffrey S (Department: 1639)
Combinatorial chemistry technology: method, library, apparatus
Method of screening a library
By measuring the effect on a living organism, tissue, or cell
C435S006120
Reexamination Certificate
active
07829503
ABSTRACT:
The present invention relates to a method for screening and identifying compounds that modulate the activity of a fungal tRNA splicing endonuclease. In particular, the invention provides assays for the identification of compounds that inhibit or reduce the activity of a fungal tRNA splicing endonuclease. The methods of the present invention provide a simple, sensitive assay for high-throughput screening of libraries of compounds to identify pharmaceutical leads useful for preventing, treating, managing and/or ameliorating a fungal infection or fungal infestation or one or more symptoms thereof.
REFERENCES:
patent: 4987071 (1991-01-01), Cech et al.
patent: 5093246 (1992-03-01), Cech et al.
patent: 5310664 (1994-05-01), Butow et al.
patent: 5354855 (1994-10-01), Cech et al.
patent: 5439797 (1995-08-01), Tsien et al.
patent: 5591610 (1997-01-01), Cech et al.
patent: 5726195 (1998-03-01), Hill et al.
patent: 5776738 (1998-07-01), Dell'Orco, Sr.
patent: 5939288 (1999-08-01), Thornburg
patent: 5994124 (1999-11-01), Bozzoni
patent: 6025167 (2000-02-01), Cech et al.
patent: 6180399 (2001-01-01), Cech et al.
patent: 6214563 (2001-04-01), Negulescu et al.
patent: 6221587 (2001-04-01), Ecker et al.
patent: 6221612 (2001-04-01), Knapp et al.
patent: 6232070 (2001-05-01), Shuman
patent: 6446032 (2002-09-01), Schimmel
patent: 6503713 (2003-01-01), Rana et al.
patent: 6583309 (2003-06-01), Rana et al.
patent: 6875736 (2005-04-01), Rana et al.
patent: 2004/0023239 (2004-02-01), Tocchini-Valentini et al.
patent: 2004/0219545 (2004-11-01), Rando et al.
patent: 2005/0053985 (2005-03-01), Trotta
patent: 2005/0142545 (2005-06-01), Conn et al.
patent: 2005/0221368 (2005-10-01), Rana et al.
patent: 2006/0194234 (2006-08-01), Conn et al.
patent: 2006/0228730 (2006-10-01), Rando et al.
patent: 2007/0020630 (2007-01-01), Trotta
patent: 2007/0178456 (2007-08-01), Trotta
patent: 1340280 (1998-12-01), None
patent: 1328156 (2001-12-01), None
patent: 0177827 (1993-11-01), None
patent: WO 91/09942 (1991-07-01), None
patent: WO 98/49274 (1998-11-01), None
patent: WO 99/20795 (1999-04-01), None
patent: WO 00/67580 (2000-11-01), None
patent: WO 01/12820 (2001-02-01), None
patent: WO 01/25486 (2001-04-01), None
patent: WO 01/53455 (2001-07-01), None
patent: WO 01/92463 (2001-12-01), None
patent: WO 02/040685 (2002-05-01), None
patent: WO 02/042326 (2002-05-01), None
patent: WO 02/083837 (2002-10-01), None
patent: WO 02/083953 (2002-10-01), None
patent: WO 04/001010 (2003-12-01), None
patent: WO 2004/087069 (2004-10-01), None
patent: WO 2004/087070 (2004-10-01), None
patent: WO 2004/087884 (2004-10-01), None
patent: WO 2005/003316 (2005-01-01), None
Abelson et al.,1998, “tRNA splicing.” Journal of Biological Chemistry 273(21):12685-12688.
Adams et al., 1991, “Fluorescence ratio imaging of cyclic AMP in single cells.” Nature 349:694-697.
Belford et al., 1993, “Multiple nucleotide cofactor use by yeast ligase in tRNA splicing. Evidence for independent ATP- and GTP-binding sites” J. Biol. Chem. 268(4):2444-2450.
Belfort, M., & Weiner, A., 1997, “Another bridge between kingdoms: tRNA splicing in archaea and eukaryotes.” Cell 89(7):1003-1006.
Bjork, G., 1995, “Biosynthesis and Function of Modified Nucleosides, in tRNA: Structure, Biosynthesis and Function.” D. Soll & U. RajBhandary (eds.), American Society for Microbiology, Washington DC: pp. 165-205.
Deutscher, M.P. , 1995 “tRNA Processing Nucleases, in tRNA:Structure, Biosynthesis and Function.” D. Soll and U. RjaBhandary (eds.), American Society for Microbiology, Washington DC: pp. 51-65.
Diener & Moore, 1998, “Solution Structure of a Substrate for the Archael Pre-tRNA Splicing Endonucleases: The Bulge-Helix-Bulge Motif.” Mol. Cell. 1:883-894.
Fruscoloni et al., 2001, “Cleavage of non-tRNA substrates by eukaryal tRNA splicing endonucleases.” EMBO Rep 2(3):217-221.
Gomes et al., 1997, “RNA splicing ligase activity in the archaeon Haloferax volcanii” Biochem & Biophys. Res. Comm. 237:588-94.
Greer et al., 1982, “Mechanism of action of a yeast RNA ligase in tRNA splicing” Cell 32:537-546.
Greer., 1986, “Assembly of tRNA Splicing Complex: Evidence for Concerted Excision and Joining Steps in Splicing In Vitro.” Mol. and Cellular. Bio., 6(2):638-642.
Hyde-Deruyscher et al., 2000, “Detection of Small-Molecule Enzyme Inhibitors with Peptides Isolated from Phage-Displayed Combinatorial Peptide Libraries.” Chem. & Biol. 7:17-25.
Ikemura, 1985, “Codon Usage and tRNA Content in Unicellular and Multicellular Organisms.” Mol. Biol. Evol., 2(1):13-34.
Ikemura, T. And Okeki, H., 1983, “Codon usage and transfer RNA contents: organism-specific codon-choice patterns in reference to the isoacceptor contents.” Cold Spring Harbor Symp. Quant. Biol. 47:1087-1097.
Kleman-Leyer et al., 1997, “Properties of H. volcanii tRNA Intron Endonuclease Reveal a Relationship between the Archaeal and Eucaryal tRNA Intron Processing Systems.” Cell., 89:839-847.
Li & Abelson, 2000, “Crystal Structure of a Dimeric Archaeal Splicing Endonuclease.” J. Mol. Biol. 302:639-648.
Li et al., 1998, “Crystal structure and evolution of a transfer RNA splicing enzyme” Science 280(5361):279-284.
Lykke-Andersen, J. & Garrett, R.A.., 1997, “RNA-protein interactions of an archaeal homotetrameric splicing endoribonuclease with an exceptional evolutionary history.” EMBO J 16(20):6290-6300.
Phizicky et al., 1986, “Saccharomyces cerevisiae tRNA ligase. Purification of the protein and isolation of the structural gene” J. of Biol. Chem. 261(6):2978-2986.
Qin & Pyle, 1999, “Site-specific labeling of RNA with fluorophores and other structural probes.” Methods 18 (1):60-70.
Reyes & Abelson 1988, “Substrate Recognition and Splice Site Determination in Yeast tRNA Splicing.” Cell, 55:719-730.
Sarkar & Hopper., 1998, “tRNA Nuclear Export inSaccharomyces cerevisiae: In Situ Hybridization Analysis.” Mol. Biol. of the Cell., 9:3041-3055.
Saxena et al., 1992, “Angiogenin is a Cytotoxic, tRNA-specific Ribonuclease in the RNase A Superfamily.” J. of Biol. Chem. 267(30):21982-21986.
Trotta et al., 1997, “The yeast tRNA splicing endonuclease: a tetrameric enzyme with two active site subunits homologous to the archaeal tRNA endonucleases.” Cell 89:849-858.
Trotta, C.R. And Abelson, J.N., 1999, “tRNA Splicing: An RNA World Add-On or an Ancient Reaction? In RNA World II” Tom Cech, Ray Gesteland and John Atkins (eds.), Cold Spring Harbor Laboratory Press, 561-584.
Trotta., 1999, “The Composition, Function and Evolution of the tRNA Splicing Endonuclease.” Thesis, California Institute of Technology, pp. 1-147.
Tsien et al., 1993, “FRET for studying intracellular signaling.” Trends in Cell. Bio. 3(7): 242-245.
Vaughn et al., 2002, “Methonine In and Out of Proteins: Targets for Drug Design.” Curr. Med. Chem. 9(3):385-409.
Volarevic et al., 2000, “Proliferation, But not Growth Blocked by Conditional Deletion of 40S Ribosomal Protein S6.” Science 288:2045-2047.
Wang et al., 1990, “Substrate Masking: Binding of RNA by EGTA-Inactivated Micrococcal Nuclease Resutls in Artifactual Inhibition of RNA Processing Reactions.” Nuc. Acids Res. 18(22):6625-6626.
Winter et al., 2000, “RNA polymerase III transcription factor TFIIIC2 is overexpressed in ovarian tumors.” Proc. Natl. Acad. Sci., 97(23):12619-12624.
Xu et al., 1990, “Purification of yeast transfer RNA ligase.” Meth. in Enzymol. 181:463-471.
Zhang et al., 1997, “Gene Expression Profiles in Normal and Cancer Cells.” Science 276:1268-1272.
Baldi et al., 1992, “Participation of the intron in the reaction catalyzed by the Xenopus tRNA splicing endonuclease.” Science 255:1404-1408.
Barbino
Boesen Christian
Jones Day
Lundgren Jeffrey S
PTC Therapeutics, Inc.
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