Methods of identifying agonists and antagonists of opioid...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S007200, C435S069100

Reexamination Certificate

active

08455683

ABSTRACT:
The invention relates generally to compositions of and methods for obtaining opioid receptor polypeptides. The invention relates as well to polynucleotides encoding opioid receptor polypeptides, the recombinant vectors carrying those sequences, the recombinant host cells including either the sequences or vectors, and recombinant opioid receptor polypeptides. By way of example, the invention discloses the cloning and functional expression of at least three different opioid receptor polypeptides. The invention includes as well, methods for using the isolated, recombinant receptor polypeptides in assays designed to select and improve substances capable of interacting with opioid receptor polypeptides for use in diagnostic, drug design and therapeutic applications.

REFERENCES:
patent: 5985600 (1999-11-01), Evans et al.
patent: 0 612 845 (1994-08-01), None
patent: WO 93/19086 (1993-09-01), None
patent: WO 94/04552 (1994-03-01), None
patent: WO 94/11500 (1994-05-01), None
Ahmed et al., Life Sciences, 44, 861-871, 1989.
Cunnungham BC and Wells JA. High-resolution epitope mapping of hGH-receptor interactions by alanine-scanning mutagenesis. Science, vol. 244, pp. 1081-1085, 1989.
George DG, et al. Current methods in sequence comparison and analysis. In Macromolecular Sequencing and Synthesis—selected methods and applications. Ed. by Schlesinger DH, Alan R. Liss, Inc, NY. pp. 127-149, 1988.
Rudinger J. Characteristics of the amino acids as components of a peptide sequence. In Peptide hormones. Ed. by Parsons JA, University Park Press, Baltimore. pp. 1-7, 1976.
Frielle T, et al. Structural basis of beta-adrenergic receptor subtype sprcificity studied with chimeric beta1/beta2-adrenergic receptors. Proc.. Natl. Acad. Sci USA, vol. 85, pp. 9494-9498, 1988.
Liggett SB, et al. Structural basis for receptor-subtype specific regulation revealed by a chimeric beta3/beta2-adrenergic receptor. Proc. Natl. Acad. Sci. USA, vol. 90, pp. 3665-3669, 1993.
Dohlman et al., “Model Systems for the Study of Seven-Transmembrane-Segment Receptors,”Annu. Rev. Biochem., 60:653-688, 1991.
Dohlman et al., “A Family of Receptors Coupled to Guanine Nucleotide Regulatory Proteins,”Biochemistry, 26:2657-2664, 1987.
Evans et al., “Cloning of a Delta Opioid Receptor by Functional Expression,”Science, 258:1952-1954, 1992.
Frielle et al., “Structural Basis of β-adrenergic Receptor Subtype Specificity Studied with Chimeric β1/β2-adrenergic Receptors,”Proc. Natl. Acad. Sci. USA, 85:9494-9498, 1988.
Gioannini, T.L. et al., “Evidence for the Presence of Disulfide Bridges in Opioid Receptors Essential for Ligand Binding. Possible Role in Receptor Activation,”J. Mol. Recogn., 2:44-48, 1989.
Kieffer et al., “The δ-opioid Receptor: Isolation of a cDNA by Expression Cloning and Pharmacological Characterization,”Proc. Natl. Acad. Sci. USA, 89:12048-12052, 1992.
Loh et al., “Molecular Characterization of Opioid Receptors,”Annu. Rev. Pharmacol. Toxicol., 30:123-147, 1990.
Lutz et al., “Opioid Receptors and Their Pharmacological Profiles,”J. Receptor Res., 12:267-286, 1992.
Mansour et al., “Anatomy of CNS Opioid Receptors,”Trends in Neurosci., 7:2445-2453, 1987.
Nock et al., “Autoradiography of [3H]U-69593 Binding Sites in Rat Brain: Evidence for K Opioid Receptor Subtypes,”Eur. J. Pharmacol., 154:27-34, 1988.
Simon, “Opioid Receptors and Endogenous Opioid Peptides,”Medicinal Res. Rev., 11:357-374, 1991.
Unterwald et al., “Neuroanatomical Localization of K1 and K2 Opioid Receptors in Rat and Guinea Pig Brain,”Brain Res., 562:57-65, 1991.
Xie et al., “Expression Cloning of cDNA Encoding a Seven-helix Receptor from Human Placenta with Affinity for Opioid Ligands,”Proc. Natl. Acad. Sci. USA, 89:4124-4158, 1992.
Yamada et al., “Cloning and Functional Characterization of a Family of Human and Mouse Somatostatin Receptors Expressed in Brain, Gastrointestinal Tract, and Kidney,”Proc. Natl. Acad. Sci. USA, 89:251-255, 1992.
Yasuda et al., “Cloning of a Novel Somatostatin Receptor, SSTR3, Coupled to Adenylylcyclase,”J. Biol. Chem., 267:20422-20428, 1992.
Schofield et al., “Molecular Characterization of a New Immunoglobulin Superfamily Protein with Potential Roles in Opioid Binding and Cell Contact,”The EMBO Journal, 8:489-495, 1989.
Probst et al., “Sequence Alignment of the G-Protein Coupled Receptor Superfamily,”DNA and Cell Biology, 11:1-20, 1992.
Fujioka et al., “Purification and Reconstitution of μ-opioid Receptors in Liposome,”Journal of Chromatography, 597:429-433, 1992.
Miller et al., “Reversed-phase Liquid Chromatographic Purification and Isolation of a Radio-iodinated Selective Probe for Mu Opioid Receptors in the Brain,”Journal of Neuroscience Methods, 41:93-99, 1992.
Shimizu, Masayoshi, “Isolation and Purification of Mu-type Opioid Receptor from Rat Brain using HPLC, and Biochemical Properties of Partially Purified Opioid Binding Protein,”Acta Scholae Medicinalis Universitatis in Gifu, 35:88-110, 1987.
Simon et al., “Purification of a Kappa-opioid Receptor Subtype from Frog Brain,”Neuropeptides, 10(1):19-28, 1987.
Ueda and Satoh, “μ-Opioid Receptor: Purification and Reconstitution with GTP-Binding Protein,”Receptor Purification, 1:115-129, 1990.
Kong, et al., “A Single Residue Aspartic Acid 95, in the δ Opioid Receptor Specifies Selective High Affinity Agonist Binding,”J. Biol. Chem., 268(31):23055-23058 (1993).
Raynor, et al., “Molecular Mechanisms of Agonist-Induced Desensitization of the Cloned MouseKappaOpioid Receptor,”J. Pharmacol. Exp. Ther., 270(3):1381-1386 (1994).
Bot et al., “Mutagenesis of the Mouse δ Opioid Receptor Converts (−)Buprenorphine From a Partial Agonist to an Antagonist,”J. Pharmacol. Exp. Ther., accepted for publication.
Blake, et al., “Differential Agonist Regulation of the Human κ-Opioid Receptor,”J. Neurochem., 68(5):1846-1852 (1997).
Dawson et al., “Chronic Exposure to κ-Opioids Enhances the Susceptibility of Immortalized Neurons (F-11κ7) to Apoptosis-Inducing Drugs by a Mechanism that May Involve Ceramide,”J. Neurochem., 68:2363-2370 (1997).
Tallent et al., “Differential Regulation of the Cloned Kappa and Mu Opioid Receptors,” submitted for publication.
Livingston et al., “Aspartate 128 in the Third Transmembrane Spanning Region of the Cloned Mouse Delta Opioid Receptor is Necessary for Agonist Binding,” initial manuscript submitted for publication.
Livingston et al., “Aspartate 128 in the Third Transmembrane Spanning Region of the Cloned MouseDeltaOpioid Receptor is Necessary for Agonist Binding,” revised manuscript submitted for publication.
Befort et al., “The Conserved Aspartate Residue in the Third Putative Transmembrane Domain of the δ-Opioid Receptor Is Not the Anionic Counterpart for Cationic Opiate Binding But Is a Constituent of the Receptor Binding Site,”Mol. Pharmacol.49:216-223 (1996).
Bot et al., “Opioid Regulation of the Mouse δ-Opioid Receptor Expressed in Human Embryonic Kidney 293 Cells,”Mol. Pharmacol.52:272-281 (1997).
Xue and Liu-Chen, “Differential Binding Domains of Peptide and Non-peptide Ligands in the Cloned Rat κ Opioid Receptor,”J. Biol. Chem.269:30195-30199 (1994).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Methods of identifying agonists and antagonists of opioid... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods of identifying agonists and antagonists of opioid..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods of identifying agonists and antagonists of opioid... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3807767

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.