Methods of generating desired amino-terminal residues in protein

Details Methods of generating desired amino-terminal residues in protein Methods of generating desired amino-terminal residues in protein

1995-06-07

1997-07-08

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4352523, 43525233, 43525411, 530350, C12N 1562, C12N 1563, C07K 14435, C12P 2104

Patent

active

056460171

ABSTRACT:
Methods of designing or modifying protein structure at the protein or genetic level to produce specified amino-termini in vivo or in vitro are described. The methods can be used to alter the metabolic stability and other properties of the protein or, alternatively, to artificially generate authentic amino-termini in proteins produced through artificial means. The methods are based upon the introduction of the use of artificial ubiquitin-protein fusions, and the discovery that the in vivo half-life of a protein is a function of the amino-terminal amino acid of the protein.

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