Methods of generating desired amino-terminal residues in protein

Details Methods of generating desired amino-terminal residues in protein Methods of generating desired amino-terminal residues in protein

1989-05-24

1992-03-03

Peet, Richard C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 691, 435 701, 435 711, 435 91, 4351721, 4351723, 4352402, 435254, 435255, 4353201, 435911, 435940, 536 27, 935 6, 935 9, 935 10, 935 22, 935 23, 935 24, 935 33, 935 34, 935 37, 935 47, 935 48, 935 49, C12N 115, C12N 510, C12N 1509, C12N 1563

Patent

active

050932420

ABSTRACT:
A method of designing or modifying protein structure at the protein or genetic level to produce specified amino-termini in vivo are described. The method can be used to alter the metabolic stability and other properties of the protein or, alternatively, to artificially generate authentic amino-termini in proteins produced through artificial means. The method is based upon the introduction of the use of artificial ubiquitin-protein fusions, and the discovery that the in vivo half-life of a protein is a function of the amino-terminal amino acid of the protein.

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