Methods of generating desired amino-terminal residues in peptide

Details Methods of generating desired amino-terminal residues in peptide Methods of generating desired amino-terminal residues in peptide

1995-12-14

1998-12-08

Degen, Nancy

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

4352402, 4352528, C07H 2104, C12N 510, C12N 120

Patent

active

058470972

ABSTRACT:
Methods of designing or modifying protein structure at the protein or genetic level to produce specified amino-termini in vivo or in vitro are described. The methods can be used to alter the metabolic stability and other properties of the protein or, alternatively, to artificially generate authentic amino-termini in proteins produced through artificial means. The methods are based upon the introduction of the use of artificial ubiquitin-protein fusions, and the discovery that the in vivo half-life of a protein is a function of the amino-terminal amino acid of the protein.

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