Methods of extracting nucleic acids and PCR amplification withou

Details Methods of extracting nucleic acids and PCR amplification withou Methods of extracting nucleic acids and PCR amplification withou

1989-10-18

1993-07-27

Moskowitz, Margaret

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 4351723, 435259, 435269, 435270, 435803, 536 221, 536 231, 935 17, 935 19, 935 78, 935 88, C12P 1934

Patent

active

052310159

ABSTRACT:
This invention provides a rapid and highly effective method for extracting nucleic acids from cells or virions without the use of proteolytic enzymes. Extraction is accomplished within a few minutes using a lysing composition comprising a buffer, a source of a DNA polymerase cofactor, a stabilizer and at least one nonionic surfactant which will release nucleic acids from cytoplasmic and nuclear membranes of cells or virions. The resulting mixture is heated to boiling for up to fifteen minutes, and the nucleic acids are recovered for amplification using polymerase chain reaction. No proteolytic enzyme is used in the extraction process.

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