Methods of extracting deoxyribonucleic acids without using a pro

Details Methods of extracting deoxyribonucleic acids without using a pro Methods of extracting deoxyribonucleic acids without using a pro

1995-06-06

1996-08-06

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435 912, 435810, 436501, 536 221, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 536 254, 935 7, 935 77, 935 78, C12P 1934, C12Q 168, C07H 2100

Patent

active

055433057

ABSTRACT:
This invention provides a rapid and highly effective method for extracting nucleic acids from cells or virions without the use of proteolytic enzymes. Extraction is accomplished within a few minutes using a lysing composition comprising a buffer, a source of a DNA polymerase cofactor, a stabilizer and at least one nonionic surfactant which will release nucleic acids from cytoplasmic and nuclear membranes of cells or virions. The resulting mixture is heated to boiling for up to fifteen minutes, and the nucleic acids are recovered for amplification using polymerase chain reaction. No proteolytic enzyme is used in the extraction process.

REFERENCES:
patent: 4750982 (1988-06-01), Tomblin et al.
patent: 4900677 (1990-02-01), Hewitt
patent: 5231015 (1993-07-01), Cummins et al.
patent: 5334499 (1994-08-01), Burdick et al.
Signer et al, Nucleic Acids Res., 16(15), p. 7738 (1988).
Higuchi, Amplifications, May, 1989, pp. 1 and 3.
Kogan et al, N. Eng. J. Med., 317(6), pp. 985-990 (1987).
Saiki et al, Nature, 324(6093), pp. 163-166 (1986).
Saiki et al, Bio/Technology, 3, pp. 1008-1012 (1985).
Bell et al, Proc. Natl. Acad. Sci. USA, 78(9), pp. 5759-5783 (1981).
Adler et al, Jun. 8, 1989, Abstract entitled "Detection of HIV-I DNA is Peripheral Blood by Polymerase Chain Reaction (PCR) and Non-Radioactive Oligonucleotide Probes".
Owen et al, Nucleic Acid Research:, 15(8), p. 3631, 1987.
Longmire et al, Nucleic Acids Research, 15(2), p. 859, 1987.
Buffone et al, Clin. CHem., 31(1), pp. 164-165 (1985).
Kolodner et al, Biochim. Biophysi Acta, 402, pp. 372-390, 1975.
Beji et al, Anal. Biochem., 162, pp. 18-23 (1987).
Blin et al, Nucl. Acids Research., 3(9), ppl 2303-2308, 1976.
Hillenbrand et al. (1982) Nucleic Acids Res., vol. 10, No. 3, pp. 838-853.
Hutchison et al, Cell, vol. 43, pp. 471-482 (1985).
Webster'S II, New Riverside Dictionary(Ed. A. Soukhanov et al., Houghton Mifften Co., Boston, MA., 1984), p. 948.

Affiliated with

Also associated with

Rating

Methods of extracting deoxyribonucleic acids without using a pro does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods of extracting deoxyribonucleic acids without using a pro, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods of extracting deoxyribonucleic acids without using a pro will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2190556